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重組蛋白

產品名稱 貨號 規格
Recombinant Escherichia coli O157:H7 Quinate/shikimate dehydrogenase (ydiB) CSB-YP471433EOE
CSB-EP471433EOE
CSB-BP471433EOE
CSB-MP471433EOE
CSB-EP471433EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP471434EOE
CSB-EP471434EOE
CSB-BP471434EOE
CSB-MP471434EOE
CSB-EP471434EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Arginine N-succinyltransferase (astA) CSB-YP471435EOE
CSB-EP471435EOE
CSB-BP471435EOE
CSB-MP471435EOE
CSB-EP471435EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA-modifying protein ygfZ (ygfZ) CSB-YP471436EOE
CSB-EP471436EOE
CSB-BP471436EOE
CSB-MP471436EOE
CSB-EP471436EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribose-5-phosphate isomerase A (rpiA) CSB-YP471437EOE
CSB-EP471437EOE
CSB-BP471437EOE
CSB-MP471437EOE
CSB-EP471437EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0301 protein yqgE (yqgE) CSB-YP471438EOE
CSB-EP471438EOE
CSB-BP471438EOE
CSB-MP471438EOE
CSB-EP471438EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP471439EOE
CSB-EP471439EOE
CSB-BP471439EOE
CSB-MP471439EOE
CSB-EP471439EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Leucine--tRNA ligase (leuS), partial CSB-YP471440EOE
CSB-EP471440EOE
CSB-BP471440EOE
CSB-MP471440EOE
CSB-EP471440EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutamine--tRNA ligase (glnS) CSB-YP471441EOE
CSB-EP471441EOE
CSB-BP471441EOE
CSB-MP471441EOE
CSB-EP471441EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0181 protein yoaH (yoaH) CSB-YP471442EOE
CSB-EP471442EOE
CSB-BP471442EOE
CSB-MP471442EOE
CSB-EP471442EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protease HtpX (htpX), partial CSB-YP471443EOE1
CSB-EP471443EOE1
CSB-BP471443EOE1
CSB-MP471443EOE1
CSB-EP471443EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Aspartate--tRNA ligase (aspS) CSB-YP471444EOE
CSB-EP471444EOE
CSB-BP471444EOE
CSB-MP471444EOE
CSB-EP471444EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0441 protein ygiB (ygiB) CSB-YP471445EOE
CSB-EP471445EOE
CSB-BP471445EOE
CSB-MP471445EOE
CSB-EP471445EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP471446EOE
CSB-EP471446EOE
CSB-BP471446EOE
CSB-MP471446EOE
CSB-EP471446EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP471447EOE
CSB-EP471447EOE
CSB-BP471447EOE
CSB-MP471447EOE
CSB-EP471447EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Flagellar transcriptional regulator FlhD (flhD) CSB-YP471448EOE
CSB-EP471448EOE
CSB-BP471448EOE
CSB-MP471448EOE
CSB-EP471448EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Uronate isomerase (uxaC) CSB-YP471449EOE
CSB-EP471449EOE
CSB-BP471449EOE
CSB-MP471449EOE
CSB-EP471449EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0213 protein yhbQ (yhbQ) CSB-YP471450EOE
CSB-EP471450EOE
CSB-BP471450EOE
CSB-MP471450EOE
CSB-EP471450EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 GTPase obg (obg) CSB-YP471451EOE
CSB-EP471451EOE
CSB-BP471451EOE
CSB-MP471451EOE
CSB-EP471451EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 PKHD-type hydroxylase ybiX (ybiX) CSB-YP471452EOE
CSB-EP471452EOE
CSB-BP471452EOE
CSB-MP471452EOE
CSB-EP471452EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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