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重組蛋白

產品名稱 貨號 規格
Recombinant Acidithiobacillus ferrooxidans S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP470782AWI
CSB-EP470782AWI
CSB-BP470782AWI
CSB-MP470782AWI
CSB-EP470782AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Peptidyl-tRNA hydrolase (pth) CSB-YP470783AWI
CSB-EP470783AWI
CSB-BP470783AWI
CSB-MP470783AWI
CSB-EP470783AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP470784AWI
CSB-EP470784AWI
CSB-BP470784AWI
CSB-MP470784AWI
CSB-EP470784AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP470785AWI
CSB-EP470785AWI
CSB-BP470785AWI
CSB-MP470785AWI
CSB-EP470785AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP470786AWI
CSB-EP470786AWI
CSB-BP470786AWI
CSB-MP470786AWI
CSB-EP470786AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP470787AWI
CSB-EP470787AWI
CSB-BP470787AWI
CSB-MP470787AWI
CSB-EP470787AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP470788AWI
CSB-EP470788AWI
CSB-BP470788AWI
CSB-MP470788AWI
CSB-EP470788AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP470789AWI
CSB-EP470789AWI
CSB-BP470789AWI
CSB-MP470789AWI
CSB-EP470789AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L24 (rplX) CSB-YP470790AWI
CSB-EP470790AWI
CSB-BP470790AWI
CSB-MP470790AWI
CSB-EP470790AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L15 (rplO) CSB-YP470791AWI
CSB-EP470791AWI
CSB-BP470791AWI
CSB-MP470791AWI
CSB-EP470791AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Serine--tRNA ligase (serS) CSB-YP470792AWI
CSB-EP470792AWI
CSB-BP470792AWI
CSB-MP470792AWI
CSB-EP470792AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L20 (rplT) CSB-YP470793AWI
CSB-EP470793AWI
CSB-BP470793AWI
CSB-MP470793AWI
CSB-EP470793AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Deoxycytidine triphosphate deaminase (dcd) CSB-YP470794AWI
CSB-EP470794AWI
CSB-BP470794AWI
CSB-MP470794AWI
CSB-EP470794AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP470795AWI
CSB-EP470795AWI
CSB-BP470795AWI
CSB-MP470795AWI
CSB-EP470795AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable intracellular septation protein A (Lferr_0816), partial CSB-YP470796AWI1
CSB-EP470796AWI1
CSB-BP470796AWI1
CSB-MP470796AWI1
CSB-EP470796AWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ketol-acid reductoisomerase (ilvC) CSB-YP470797AWI
CSB-EP470797AWI
CSB-BP470797AWI
CSB-MP470797AWI
CSB-EP470797AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP470798AWI
CSB-EP470798AWI
CSB-BP470798AWI
CSB-MP470798AWI
CSB-EP470798AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Sugar fermentation stimulation protein homolog (sfsA) CSB-YP470799AWI
CSB-EP470799AWI
CSB-BP470799AWI
CSB-MP470799AWI
CSB-EP470799AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UPF0042 nucleotide-binding protein Lferr_2629 (Lferr_2629) CSB-YP470800AWI
CSB-EP470800AWI
CSB-BP470800AWI
CSB-MP470800AWI
CSB-EP470800AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP470801AWI
CSB-EP470801AWI
CSB-BP470801AWI
CSB-MP470801AWI
CSB-EP470801AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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