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GST Monoclonal Antibody

  • 中文名稱:
    GST鼠單克隆抗體
  • 貨號:
    CSB-MA000031M1m
  • 規(guī)格:
    ¥400
  • 圖片:
    • Western Blot
      Positive WB detected in: 25ng Recombinant protein with GST tag from E.coli
      All lanes: GST antibody at 1:10000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDa
      Observed band size: 31, 30, 31, 37, 30, 60, 37, 61 KDa
      Exposure time:5min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli
      All lanes: GST antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 26, 102.9, 75.6 KDa
      Observed band size: 26, 103, 76 KDa
      Exposure time:1min
    • Western Blot
      Positive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
      All lanes: GST antibody at 1:10000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 26 KDa
      Observed band size: 26 KDa
      Exposure time:5min
    • Western Blot
      Positive WB detected in: 25ng recombinant protein with GST tag from E.coli
      GST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 26 KDa
      Observed band size: 26 KDa
      Exposure time:5min
    • Immunofluorescence staining of 293F transfected cells with CSB-MA000031M1m at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
    • Immunoprecipitating GST in transfected-293F whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000031M1m in 293F cell lysate Transfected with GST
      Lane 2: CSB-MA000031M1m (5μg) + Transfected-293F whole cell lysate (500μg)
      Lane 3: Transfected-293F whole cell lysate (10μg)
      For western blotting, the blot was detected with CSB-MA000031M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
    • Overlay Peak curve showing 293F cells transfected with GST stained with CSB-MA000031M1m (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他:

產品詳情

  • 產品描述:
    本產品為高特異性GST單克隆抗體(CUSABIO貨號:CSB-MA000031M1m),靶向谷胱甘肽S-轉移酶(GST),該蛋白作為重要的Ⅱ相代謝酶,在細胞解毒、藥物代謝及氧化應激調控等研究中具有關鍵作用。該抗體具有廣譜物種反應性,適用于ELISA、Western Blot、免疫熒光、流式細胞術及免疫共沉淀等多種實驗體系,可精準識別天然及重組GST蛋白。其高親和力特性在重組蛋白純化驗證、GST融合蛋白相互作用分析、代謝通路研究等科研場景中表現優(yōu)異,特別適用于藥物開發(fā)中代謝酶活性檢測及毒理學研究。經嚴格質控驗證,該抗體在多種實驗條件下均保持穩(wěn)定性能,為腫瘤機制探索、環(huán)境毒物代謝分析等基礎研究提供可靠工具。作為科研專用試劑,本產品廣泛應用于分子生物學、細胞生物學及生物化學領域,是研究蛋白質相互作用網絡和代謝調控機制的重要工具抗體。
  • 基因名:
    GST
  • 別名:
    Glutathione S-Transferase, gst, GST tag
  • 宿主:
    Mouse
  • 反應種屬:
    All
  • 免疫原:
    Recombinant GST Protein
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    IgG2b
  • 純化方式:
    >95%, Protein A purified
  • 克隆號:
    3D3E4
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IF, FC, IP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:10000-1:640000
    IF 1:100-1:300
    FC 1:100-1:300
    IP 1ul-5ul
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價



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