GFP Monoclonal Antibody
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中文名稱:GFP鼠單克隆抗體
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貨號(hào):CSB-MA000051M1m
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規(guī)格:¥400
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圖片:
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Western Blot
Positive WB detected in: 50ng recombinant protein
All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time:5min -
Western Blot
Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng
All lanes: GFP antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time:5min -
Western Blot
Positive WB detected in: 1-4 lanes:Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5μg
All lanes GFP antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively
Observed band size: 32,32,50,32,32 KDa
Exposure time:1min -
Immunofluorescence staining of 293F cells transfected with GFP with CSB-MA000051M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
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Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
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Overlay histogram showing 293F cells untransfected (green line) or transfected with GFP (red line) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
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Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP
Lane 1: Mouse control IgG2b instead of CSB-MA000051M1m in 293F whole cell lysate transfected with GFP
Lane 2: CSB-MA000051M1m (4μg) + 293F whole cell lysate transfected with GFP (500μg)
Lane 3: 293F whole cell lysate transfected with GFP (5μg)
For western blotting, the blot was detected with CSB-MA000051M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:本產(chǎn)品為GFP單克隆抗體(CUSABIO貨號(hào):CSB-MA000051M1m),專為檢測(cè)綠色熒光蛋白(GFP)設(shè)計(jì)。GFP作為分子生物學(xué)中廣泛應(yīng)用的熒光標(biāo)記蛋白,其特異性檢測(cè)在基因表達(dá)示蹤、蛋白定位分析及細(xì)胞功能研究中具有重要價(jià)值。該抗體通過(guò)ELISA、Western Blot(WB)、免疫熒光(IF)、流式細(xì)胞術(shù)(FC)和免疫沉淀(IP)五大實(shí)驗(yàn)平臺(tái)驗(yàn)證,適用于轉(zhuǎn)基因細(xì)胞株篩選、重組蛋白表達(dá)驗(yàn)證、熒光融合蛋白追蹤等科研場(chǎng)景,展現(xiàn)廣泛的物種兼容性。其高靈敏度和特異性可精準(zhǔn)識(shí)別天然GFP及其衍生變體(如EGFP、YFP),在活細(xì)胞成像、蛋白質(zhì)相互作用研究及報(bào)告基因系統(tǒng)中表現(xiàn)卓越。作為科研級(jí)試劑,本產(chǎn)品采用高純度雜交瘤技術(shù)制備,批次穩(wěn)定性優(yōu)異,為熒光標(biāo)記實(shí)驗(yàn)提供可靠工具,助力基因編輯、細(xì)胞信號(hào)通路及亞細(xì)胞結(jié)構(gòu)研究。核心關(guān)鍵詞:GFP單克隆抗體、綠色熒光蛋白檢測(cè)、免疫印跡抗體、流式細(xì)胞術(shù)試劑、免疫熒光探針、重組蛋白檢測(cè)。
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基因名:GFP
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別名:CFP, eGFP, eYFP, GFP, GFP tag, YFP
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宿主:Mouse
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反應(yīng)種屬:N/A
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免疫原:Recombinant GFP Protein
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標(biāo)記方式:Non-conjugated
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克隆類型:Monoclonal Antibody
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抗體亞型:IgG2b
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純化方式:>95%,Protein A purified
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克隆號(hào):6C11C11
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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產(chǎn)品提供形式:liquid
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應(yīng)用范圍:ELISA, WB, IF, FC, IP
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推薦稀釋比:
Application Recommended Dilution WB 1:50000-1:6400000 IF 1:50-1:200 FC 1:100-1:300 IP 1μl-2μl -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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