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Avi-Tag Monoclonal Antibody

  • 中文名稱：
    Avi-Tag鼠單克隆抗體
  • 貨號：
    CSB-MA000101M0m
  • 規格：
    ￥400
  • 圖片：
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cell All lanes: Avi tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
      Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli All lanes: Avi tag antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 81.2, 67, 66.9, 66.9 KDa
      Observed band size: 82, 67, 67, 67KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: Avi tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40 KDa
      Observed band size: 40 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40 KDa
      Observed band size: 40 KDa
      Exposure time：5min
    • Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
      Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag. Lane 2: CSB-MA000101M0m (1μg) + 293F whole cell lysate transfected with avi tag (500μg)
      Lane 3: 293F whole cell lysate transfected with avi tag(40μg)
      For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000
  • 其他：

產品詳情

  • 產品描述：
    CUSABIO貨號：CSB-MA000101M0m Avi-Tag單克隆抗體是針對生物素化標簽系統開發的高特異性科研試劑，其靶向的AVI標簽是廣泛應用于重組蛋白標記領域的15個氨基酸短肽序列，通過與鏈霉親和素的高親和力結合，顯著提升蛋白純化效率及檢測靈敏度。本產品采用雜交瘤技術制備，具備嚴格的批間一致性，經實驗驗證可特異性識別AVI標簽而不與天然生物素發生交叉反應，適用于ELISA、Western Blot和免疫沉淀（IP）三大核心蛋白研究技術平臺。在重組蛋白表達驗證實驗中，該抗體能有效檢測哺乳動物、昆蟲等多種表達系統產生的AVI標記蛋白；在蛋白互作研究中，可通過IP實驗高效富集標記蛋白復合物；其Western Blot應用可精準識別1-100 kDa分子量范圍內的標記靶蛋白。該抗體已通過多種物種樣本驗證，為基因編輯驗證、蛋白互作組學研究和體外診斷試劑開發提供關鍵工具，特別適用于需要高靈敏度檢測的生物制藥研發、蛋白質工程優化等生命科學領域。本產品嚴格遵循科研試劑標準，配套提供詳細技術參數及實驗方案，助力研究人員實現精準高效的AVI標記蛋白檢測與功能解析。
  • 產品名稱：
    Mouse anti-All AVI Monoclonal antibody
  • 基因名：
    AVI
  • 宿主：
    Mouse
  • 反應種屬：
    All
  • 免疫原：
    Synthetic Peptide
  • 免疫原種屬：
    All
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2a
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    9G3B5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:640000
    IP 2μl-5μl
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價



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