Myc tag Monoclonal Antibody
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中文名稱：Myc tag鼠單克隆抗體
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貨號：CSB-MA000041M0m
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規(guī)格：￥400
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圖片：
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WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
Lane 1: Recombinant Myc-tagged fusion protein at 50ng
Lane 2: Recombinant Myc-tagged fusion protein at 25ng
Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50 kd
Observed band size: 50 kd -
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
Lane 3: Transfected HEK293 whole cell lysate (10µg)
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA000041M0m Myc標(biāo)簽單克隆抗體是針對Myc表位標(biāo)簽研發(fā)的高特異性科研試劑，該抗體采用雜交瘤技術(shù)制備，具有批次穩(wěn)定性和優(yōu)異的抗原結(jié)合能力。Myc標(biāo)簽作為分子生物學(xué)研究中廣泛應(yīng)用的短肽標(biāo)簽（EQKLISEEDL），常被用于重組蛋白表達(dá)系統(tǒng)的追蹤、純化及功能驗(yàn)證。本產(chǎn)品通過嚴(yán)格驗(yàn)證，適用于Western Blot、免疫熒光、免疫沉淀及ELISA等多種實(shí)驗(yàn)體系，可精準(zhǔn)識別C端或N端融合的Myc標(biāo)簽蛋白。其廣譜物種兼容性覆蓋哺乳動物、昆蟲及酵母等表達(dá)系統(tǒng)，特別適用于重組蛋白表達(dá)驗(yàn)證、亞細(xì)胞定位分析以及蛋白相互作用研究等實(shí)驗(yàn)場景。該抗體經(jīng)多次親和純化處理，在1:1000工作濃度下展現(xiàn)出清晰的檢測信號與低背景特性，是蛋白質(zhì)組學(xué)研究和基因功能分析的關(guān)鍵工具試劑。實(shí)驗(yàn)方案推薦使用含5%脫脂牛奶的TBST封閉液進(jìn)行膜封閉，搭配辣根過氧化物酶標(biāo)記二抗實(shí)現(xiàn)可視化檢測，助力科研人員高效完成分子互作機(jī)制、信號通路解析等前沿課題研究。
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別名：bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
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宿主：Mouse
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反應(yīng)種屬：N/A
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免疫原：EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號：5F92F9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:100-1:300 IP 1:1000-1:1500 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻(xiàn)
- Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex Abad M A, et al,The Journal of Cell Biology,2019
- The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation Jheng JR.et al,J Cachexia Sarcopenia Muscle,2018
產(chǎn)品評價(jià)
問答及客戶評論
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Western blot review for CD146 Monoclonal AntibodyTested application: WB
Test sample: Cells
Sample volume: 4μg
Electrophoresis parameters: 100V
Primary antibody dilution ratio: 1:500
Review: Antibody specificity is very good. -
Western blot review for CD146 Monoclonal AntibodyTested application: WB
Test sample: HEK293 cell lysate
Sample volume: 20μg
Electrophoresis parameters: 80V/100V
Primary antibody dilution ratio: 1:1000
Review: Myc antibody has good specificity.
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