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  4. Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4)

Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4)

  • 中文名稱：
    Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4)
  • 品名簡稱：
    Recombinant Tomato bushy stunt virus ORF4 protein
  • 貨號：
    CSB-EP320894TGA
  • 說明書：
  • 規格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    ORF4
  • Uniprot No.：
  • 別名：
    19 kDa symptom severity modulator
  • 種屬：
    Tomato bushy stunt virus (strain Cherry) (TBSV)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    26.0 kDa
  • 表達區域：
    1-172aa
  • 氨基酸序列
    MERAIQGNDAREQANSERWDGGSGGTTSPFKLPDESPSWTEWRLHNDETNSNQDNPLGFKESWGFGKVVFKRYLRYDRTEASLHRVLGSWTGDSVNYAASRFFGFDQIGCTYSIRFRGVSITVSGGSRTLQHLCEMAIRSKQELLQLAPIEVESNVSRGCPEGTETFEKESE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-tagged and C-terminal CBD-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.
  • 基因功能參考文獻：
    1. Both observed variation and potential variation (where a non-synonymous change to p19 would be synonymous for the overprinted movement protein) does not significantly impact protein structure or RNA binding. PMID: 26871901
    2. Data indicate that p19 exhibits size-specific and sequence-independent binding of its small RNA ligand. PMID: 23376479
    3. The results demonstrate that transient co-transformation of plant cell suspension culture with two transformation vectors is feasible and that the use of the viral suppressor of silencing p19 significantly raises the production of the protein of interest. PMID: 20859930
    4. involved in suppression of posttranscriptional gene silencing, virus movement, and symptom induction. PMID: 15299117
    5. activity hindered by defective interfering RNA PMID: 15596838
    6. This is the first clear demonstration of a direct correlation between a novel structurally orchestrated siRNA binding of an RNAi suppressor, P19 protein, and its roles in viral pathogenesis. PMID: 16501109
    7. Substitutions on P19 that compromised siRNA binding not only affected systemic invasion and symptom development but also resulted in newly recognized effects on the integrity of virus and host material in a plant species-dependent manner. PMID: 19052093
    8. P19 suppresses a common step in RNA silencing (innate immunity) that is downstream of small RNA maturation PMID: 19122141

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  • 蛋白家族：
    Tombusviruses protein p19 family
  • 數據庫鏈接：

    KEGG: vg:1493957