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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase STE20 (STE20), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase STE20 (STE20), partial

  • 中文名稱：
    釀酒酵母STE20重組蛋白
  • 貨號：
    CSB-YP311785SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母STE20重組蛋白
  • 貨號：
    CSB-EP311785SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母STE20重組蛋白
  • 貨號：
    CSB-EP311785SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母STE20重組蛋白
  • 貨號：
    CSB-BP311785SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母STE20重組蛋白
  • 貨號：
    CSB-MP311785SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    STE20
  • Uniprot No.：
  • 別名：
    STE20; YHL007C; Serine/threonine-protein kinase STE20; EC 2.7.11.1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.
  • 基因功能參考文獻：
    1. during filamentous growth, Ste20 stimulates V-ATPase activity, this would sustain nutrient mobilization from vacuolar stores, which is beneficial for filamentous growth. PMID: 22327006
    2. cell cycle kinases Ste20 as central mediators of the cellular response to inhibition of fatty acid synthesis. (Ste20) PMID: 22218487
    3. An in silico predictor was built to identify Ste20p substrates on a proteome-wide basis. PMID: 20020052
    4. An evolutionarily conserved binding site for Bem1 between the CRIB and kinase domains of Ste20 was identified. PMID: 15743816
    5. In mitogen-activated protein kinase (MAPK) cascades of budding yeast, pheromone-induced mating signal is transmitted by interactions between the beta-subunit of a G-protein (G-beta) and the G-beta binding (GBB) domain of Ste20 kinase. PMID: 16870141
    6. to gain further insight into the mechanisms of Ste20 action in cell polarity, we screened for interactors of Ste20; among the identified proteins were Erg4, Cbr1 and Ncp1, which are all involved in sterol biosynthesis PMID: 17895367

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=The translocation from the cytoplasm to the nucleus is stimulated by H(2)O(2). Localizes at bud emergence during cell cycle and the shmoo top during mating, both localizations requiring an interaction with CDC42.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
  • 數據庫鏈接：

    KEGG: sce:YHL007C

    STRING: 4932.YHL007C



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