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  4. Recombinant Rat Spectrin alpha chain, brain (Sptan1), partial

Recombinant Rat Spectrin alpha chain, brain (Sptan1), partial

  • 中文名稱：
    大鼠Sptan1重組蛋白
  • 貨號：
    CSB-YP022633RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Sptan1重組蛋白
  • 貨號：
    CSB-EP022633RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Sptan1重組蛋白
  • 貨號：
    CSB-EP022633RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Sptan1重組蛋白
  • 貨號：
    CSB-BP022633RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Sptan1重組蛋白
  • 貨號：
    CSB-MP022633RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Sptan1; Spna2; Spta2; Spectrin alpha chain; non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

Regarding your protein c, could you please advise on the pricing, the lead time, the sequencing, and the expected tag information for all 4 expression hosts?
I'm interested in the full-length, but I told them that a full-length probably wouldn’t be possible, even as a custom project, so if there was a smaller region they were interested in, I told them to suggest one. They said the first half of the protein would be nice as well, but that is still very large – how big do you think would be a suitable size your team would be comfortable trying to express?

A:
Very nice to receive your inquiry. We've expressed the human SPTAN1 successfully. Here is the detail information.
Recombinant Human Spectrin alpha chain, brain(SPTAN1) ,partial
CSB-EP022633HU
Expression Region: 1-580aa；Partial protien at N-terminus
Tag information: N-terminal 6xHis-SUMO-tagged
Sequence:

MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELASDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTVLSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMRRRAQLADSFHLQQFFR


It is high homologous(98%) between human and rat. So that the success rate is quite high. Here is the quotation of rat SPTAN1.
Recombinant Rat Spectrin alpha chain, brain(Sptan1) ,partial
CSB-YP022633RA
CSB-EP022633RA
CSB-BP022633RA
CSB-MP022633RA
Expression Region: 1-580aa；Partial protien at N-terminus
Tag information: :EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
Sequence:

MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAKLIQEQHPEEELIKTKQEEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSANQIQVKREELITNWEQIRTLAAERHARLDDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLSILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMHRRAQLADSFHLQQFFR

靶點詳情

  • 功能：
    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • 基因功能參考文獻：
    1. These findings reveal spectrin (alpha/beta) / ankyrin B cytoskeletal and signaling proteins as key regulators of T-type calcium channels expressed in the nervous system. PMID: 29720258
    2. The present study demonstrates the age-dependent intraneuronal presence of an alphaII-spectrin cleavage fragment in mammalian forebrain which is exacerbated in a transgenic model of AD. PMID: 22723836
    3. an aberrant decrease in inhibitory protein factor (IPF) is associated with certain forms of seizure [IPF] PMID: 12350384
    4. Results indicate that phosphoinositide 3-kinase might accelerate hypoxic cell death by enhancing the calpain-dependent proteolysis of fodrin. PMID: 12473194
    5. alphaII-spectrin breakdown products detected in CSF after injury, particularly those mediated by calpain, may be useful diagnostic indicators of cerebral infarction PMID: 14688621
    6. The interaction between alpha II-spectrin SH3 domain and EVL protein suggests a new way of integrating the spectrin-based skeleton in areas of dynamic actin reorganization. PMID: 16336193
    7. We show that fodrin interacts with the t-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) syntaxin 4, and this interaction is increased by insulin stimulation and decreased by prior latrunculin A treatment. PMID: 16870704
    8. Distinct proteolytic products resulting from the degradation of the cytoskeletal protein alpha-II-spectrin by calpain and caspase 3 were readily detectable in cortical brain parenchyma and cerebrospinal fluid after TBI in immature rats. PMID: 16943668
    9. support a critical role for the alphaII-spectrin-SH3i isoform of spectrin in intracellular targeting of connexin 43 to gap junctions PMID: 17276456
    10. PDE4D4 interacts with alpha II spectrin within this membrane domain. PMID: 18073242

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • 蛋白家族：
    Spectrin family
  • 組織特異性：
    Expressed in the foot process layer of podocytes in the kidney glomerulus and in tubules (at protein level).
  • 數據庫鏈接：

    KEGG: rno:64159

    STRING: 10116.ENSRNOP00000042382

    UniGene: Rn.5812