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  4. Recombinant Pseudomonas putida Probable HTH-type transcriptional regulator ttgR (ttgR)

Recombinant Pseudomonas putida Probable HTH-type transcriptional regulator ttgR (ttgR)

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  • 中文名稱：
    Recombinant Pseudomonas putida Probable HTH-type transcriptional regulator ttgR (ttgR)
  • 品名簡稱：
    Recombinant Pseudomonas putida ttgR protein
  • 貨號：
    CSB-EP801612FGB
  • 說明書：
  • 規格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    ttgR
  • Uniprot No.：
  • 別名：
    Efflux pump ttgABC operon regulator
  • 種屬：
    Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    30.7 kDa
  • 表達區域：
    1-210aa
  • 氨基酸序列
    MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDELDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDVERAAVAMFAYVDGLIGRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Probable regulatory protein for the ttgABC efflux pump operon. May function as a repressor.
  • 基因功能參考文獻：
    1. The data provide new information and deepen the understanding of the TtgR-effector binding mechanism and consequently the TtgABC efflux pump regulation mechanism in Pseudomonas putida. PMID: 26422008
    2. analysis of protein domain cross-talk during effector binding to the multidrug binding TTGR regulator PMID: 20435893
    3. the binding of a single effector molecule to the DNA-bound TtgR homodimer induces the dissociation of the repressor-operator complex PMID: 16407274
    4. a model for the binding of TtgR to its target operator sequence PMID: 17498746
  • 數據庫鏈接：

    KEGG: ppu:PP_1387

    STRING: 160488.PP_1387