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Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxB (esxB)

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  • 中文名稱:
    結核分枝桿菌esxB重組蛋白
  • 品名簡稱:
    Recombinant Mycobacterium tuberculosis esxB protein
  • 貨號:
    CSB-EP363658MVZ
  • 說明書:
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    esxB
  • Uniprot No.:
  • 別名:
    esxB; cfp10; lhp; mtsA10; MT3988ESAT-6-like protein EsxB; 10 kDa culture filtrate antigen CFP-10; Secreted antigenic protein MTSA-10
  • 種屬:
    Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    14.7
  • 表達區域:
    2-100aa
  • 氨基酸序列
    AEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Tris-based buffer,50% glycerol
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    This recombinant M.tb esxB protein is an E.coli?expressed protein (Full Length of Mature Protein) with N-terminal 6xHis tag?and its purity is 90%+ determined by SDS-PAGE. With the appropriate cDNA and PCR methods, esxB?expression plasmids can be rapidly produced. which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, esxB proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, esxB proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.

    esxB, also called CFP-10, is a Mycobacterium tuberculosis secreted antigen that contributes to viral virulence and pathogenicity. CFP-10 and ESAT-6 forms a 1:1 heterodimeric complex that plays a critical role in the pathogenesis of tuberculosis (TB). Amanda Welin et al. has shown that the ESAT-6/CFP-10 complex induces a transient release of Ca2+ from intracellular stores in human neutrophils. CFP-10 rather than ESAT-6 was responsible for triggering the Ca2+ response, in a pertussis toxin-sensitive manner, implying the participation of a GPCR.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    WXG100 family, CFP-10 subfamily
  • 數據庫鏈接:

    KEGG: mtc:MT3988



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