Recombinant Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat)

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中文名稱：

小鼠Dlat重組蛋白
品名簡稱：

Recombinant Mouse Dlat protein
貨號：

CSB-EP804374MOa0
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP804374MOa0 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Dlat.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP804374MOa0 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Dlat.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Dlat
Uniprot No.：

Q8BMF4
別名：

Dlat; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial; EC 2.3.1.12; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

62.8 kDa
表達區(qū)域：

86-642aa
氨基酸序列

SLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDLAAAAAPQAAPAAAPAPAAAPAAPSASAPGSSYPTHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGAPLCIIVEKQEDIAAFADYRPTEVTSLKPQAAPPAPPPVAAVPPTPQPVAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPSKAAPAAAAAMAPPGPRVAPAPAGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPITMLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer，50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

DLAT是線粒體內(nèi)的一種蛋白質(zhì)，它是丙酮酸脫氫酶復合物（Pyruvate Dehydrogenase Complex，簡稱 PDC）的一部分，負責催化乙酰輔酶A的合成。PDC是一種分布于線粒體基質(zhì)中的多酶復合體，通過催化丙酮酸的氧化脫羧反應(yīng)生成乙酰輔酶A，有效連接糖酵解和三羧酸循環(huán)。在科研上，DLAT的重組蛋白被廣泛用于各種研究中。例如，它被用于加速信號傳導研究，因為它是細胞內(nèi)復雜世界的關(guān)鍵組成部分。此外，DLAT的重組蛋白也被用于研究腫瘤免疫逃逸，因為研究發(fā)現(xiàn)，丙酮酸脫氫酶復合物E1組分α亞基（PDHE1α）存在豐富的胞質(zhì)定位，并且在腫瘤免疫逃逸中起著重要的作用。
華美生物提供大腸桿菌表達系統(tǒng)制備的全長DLAT重組蛋白，可幫助您更好地理解細胞內(nèi)的復雜機制，有望為疾病的治療提供新的思路和方法。
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
基因功能參考文獻：
1. These data reveal a central role for Dlat in the metabolic program regulated by E4F1 in basal keratinocytes. PMID: 27621431
2. On the basis of our ChIP data and these previous findings, we hypothesize that PDC may modulate STAT5's ability to regulate gene expression by controlling histone or STAT5 acetylation PMID: 28982698
3. breach of tolerance to PDC-E2 is probably the first event in the natural history of primary biliary cirrhosis in genetically susceptible hosts. PMID: 24128311
4. a novel function of pyruvate dehydrogenase complex E2 in the nucleus in up-regulating the transactivating ability of STAT5 PMID: 21397011
亞細胞定位：

Mitochondrion matrix.
蛋白家族：

2-oxoacid dehydrogenase family
數(shù)據(jù)庫鏈接：

KEGG: mmu:235339

STRING: 10090.ENSMUSP00000034567

UniGene: Mm.285076