Recombinant Mouse Cullin-7 (Cul7), partial

中文名稱：

Recombinant Mouse Cullin-7(Cul7) ,partial
貨號：

CSB-YP851837MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Cullin-7(Cul7) ,partial
貨號：

CSB-EP851837MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Cullin-7(Cul7) ,partial
貨號：

CSB-EP851837MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Cullin-7(Cul7) ,partial
貨號：

CSB-BP851837MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Cullin-7(Cul7) ,partial
貨號：

CSB-MP851837MO
規(guī)格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Cul7
Uniprot No.：

Q8VE73
別名：

Cul7; Kiaa0076Cullin-7; CUL-7; p185; p193
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5. Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1. Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development. Does not promote polyubiquitination and proteasomal degradation of p53/TP53. While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation.
基因功能參考文獻：
1. Cul7-/- mouse embryonic fibroblasts displayed an increase in the activation of AKT and Erk phosphorylation upon insulin stimulation. PMID: 24219910
2. role of binding of Cul7 to simian virus 40 large T antigen in cell transformation; may also regulate an important growth control pathway PMID: 14990695
3. Dimerization o Cul7 and Parc is not required for all Cul7 functions and mouse embryonic development. PMID: 15964813
4. suggested a role for p193/CUL7 in the regulation of apoptosis independently of p53 and Parc activity PMID: 17229476
5. Results demonstrate a key role for the CUL7 E3 in targeting IRS-1 for degradation, a process that may contribute to the regulation of cellular senescence. PMID: 18498745
6. Expression of the dominant interfering p193 transgene results in a decrease in infarct scar expansion and preservation of myocardial function at 4 weeks after Myocardial Infarction. PMID: 19435717
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亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, perinuclear region. Golgi apparatus.
蛋白家族：

Cullin family
數(shù)據(jù)庫鏈接：

KEGG: mmu:66515

STRING: 10090.ENSMUSP00000049128

UniGene: Mm.329078