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  4. Recombinant Escherichia coli Ribosome-recycling factor (frr)

Recombinant Escherichia coli Ribosome-recycling factor (frr)

  • 中文名稱：
    Recombinant Escherichia coli Ribosome-recycling factor (frr)
  • 品名簡稱：
    Recombinant E.coli frr protein
  • 貨號：
    CSB-EP014941ENV
  • 說明書：
  • 規格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    frr
  • Uniprot No.：
  • 別名：
    Ribosome-releasing factor
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    27.6 kDa
  • 表達區域：
    1-185aa
  • 氨基酸序列
    MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEYYGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPLPPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDVQKLTDAAIKKIEAALADKEAELMQF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.133.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.
  • 基因功能參考文獻：
    1. Ribosome recycling after peptide chain termination is a special case of the general RRF and EF-G function, which happens whenever fMet-tRNA is not available for triggering 70S scanning. PMID: 27261258
    2. Data show that monosomes produced in ribosome recycling factor-deficient cells are able to translate synthetic homopolymer but not natural mRNA with leader and canonical initiation signal. PMID: 15522083
    3. data enable a model to be proposed for the dual action of EF-G and RRF during ribosome recycling PMID: 15616575
    4. domain II of RRF plays a crucial role in the concerted action of RRF and EF-G for the post-termination complex disassembly, and the specific interaction between RRF and EF-G on ribosomes mainly depends on the interaction between domain II of RRF and EF-G PMID: 16262604
    5. V117D mutation does not perturb the efficient interdomain interaction, which results in cooperative unfolding of the RRF protein. PMID: 16684502
    6. RRF is capable of spontaneously moving from its initial binding site on the 70S ribosome to a site exclusively on the large 50S ribosomal subunit. PMID: 17643374
    7. The disruption of bridge B2a upon RRF binding to the ribosome seen in the present structures reveals one of the key roles that RRF plays in ribosome recycling, the dissociation of 70S ribosomes into subunits. PMID: 18234219

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    RRF family
  • 數據庫鏈接：