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  4. Recombinant Escherichia coli Nitrate/nitrite response regulator protein NarL (narL)

Recombinant Escherichia coli Nitrate/nitrite response regulator protein NarL (narL)

  • 中文名稱：
    大腸桿菌narL重組蛋白
  • 貨號：
    CSB-YP360208ENV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌narL重組蛋白
  • 貨號：
    CSB-EP360208ENV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌narL重組蛋白
  • 貨號：
    CSB-EP360208ENV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌narL重組蛋白
  • 貨號：
    CSB-BP360208ENV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌narL重組蛋白
  • 貨號：
    CSB-MP360208ENV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    narL
  • Uniprot No.：
  • 別名：
    narL; frdR; b1221; JW1212; Nitrate/nitrite response regulator protein NarL
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-216
  • 氨基酸序列
    MSNQEPATIL LIDDHPMLRT GVKQLISMAP DITVVGEASN GEQGIELAES LDPDLILLDL NMPGMNGLET LDKLREKSLS GRIVVFSVSN HEEDVVTALK RGADGYLLKD MEPEDLLKAL HQAAAGEMVL SEALTPVLAA SLRANRATTE RDVNQLTPRE RDILKLIAQG LPNKMIARRL DITESTVKVH VKHMLKKMKL KSRVEAAVWV HQERIF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.
  • 基因功能參考文獻：
    1. The NarL receiver domain operates as an on-off switch to occlude or release the output domain in a nitrate-responsive manner. PMID: 26307095
    2. NarL and NarP carboxyl-terminal domains activate transcription from Nar class II control regions. PMID: 20634237
    3. Data describe two cocrystal structures of NarL complexed with additional 20mer oligonucleotides representative of other high-affinity tail-to-tail NarL binding sites found in upstream promoter regions. PMID: 16262254
    4. binding of NarL/NarP appears to insulate the nrf promoter from the effects of integration host factor PMID: 16936015
    5. DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interac PMID: 17965164
    6. Analysis of the DNA sequence of the ydhY promoter region (PydhY) revealed the presence of four heptameric sequences similar to NarL/P binding sites PMID: 18227264
    7. DNase I footprinting studies identified Fis-binding sites that overlap the yeaR promoter NarL site at position -43.5, and the ogt promoter NarL site at position -78.5, and suggest that Fis represses both promoters by displacing NarL. PMID: 19245365
    8. The authors show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. PMID: 19251855

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