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  4. Recombinant Enterobacteria phage lambda Endopeptidase (Rz), partial

Recombinant Enterobacteria phage lambda Endopeptidase (Rz), partial

  • 中文名稱：
    Recombinant Enterobacteria phage lambda Endopeptidase(Rz),partial
  • 貨號：
    CSB-YP360436ECW
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Endopeptidase(Rz),partial
  • 貨號：
    CSB-EP360436ECW
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Endopeptidase(Rz),partial
  • 貨號：
    CSB-EP360436ECW-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Endopeptidase(Rz),partial
  • 貨號：
    CSB-BP360436ECW
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Endopeptidase(Rz),partial
  • 貨號：
    CSB-MP360436ECW
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rz
  • Uniprot No.：
  • 別名：
    RzSpanin; inner membrane subunit; i-spanin; Lysis protein Rz
  • 種屬：
    Enterobacteria phage lambda (Bacteriophage lambda)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the spanin complex that disrupts the host outer membrane and participates in cell lysis during virus exit. The spanin complex conducts the final step in host lysis by disrupting the outer membrane after holin and endolysin have permeabilized the inner membrane and degraded the host peptidoglycans. Host outer membrane disruption is due to local fusion between the inner and outer membrane performed by the spanin complex.
  • 基因功能參考文獻：
    1. When coding sequences are aligned respective to the embedded genetic architecture of Rz1 within Rz, genetically silent domains of Rz1 correspond to mutationally sensitive domains in Rz, and vice versa, suggesting that the modular structure of the two subunits facilitated the evolutionary compression that resulted in the unique embedded gene architecture. PMID: 28040784
    2. Suppressors of non-functional Rz and Rz1 mutations largely encoded polar insertions within the hydrophobic core of the coiled-coil interface. PMID: 28468876
    3. A model is proposed in which the spanins carry out the essential step of disrupting the outer membrane, in a manner regulated by the state of the peptidoglycan layer. PMID: 22904283
    4. Here the authors present evidence that an Rz-Rz1 complex that spans the periplasm carries out the final step in the process of host lysis. PMID: 18713319
  • 亞細胞定位：
    Host cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
  • 蛋白家族：
    Caudovirales i-spanin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:2703481