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  4. Recombinant Drosophila melanogaster Protein piwi (piwi), partial

Recombinant Drosophila melanogaster Protein piwi (piwi), partial

  • 中文名稱：
    黑腹果蠅piwi重組蛋白
  • 貨號：
    CSB-YP894705DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅piwi重組蛋白
  • 貨號：
    CSB-EP894705DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅piwi重組蛋白
  • 貨號：
    CSB-EP894705DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅piwi重組蛋白
  • 貨號：
    CSB-BP894705DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅piwi重組蛋白
  • 貨號：
    CSB-MP894705DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    piwi
  • Uniprot No.：
  • 別名：
    piwi; CG6122; Protein piwi; EC 3.1.26.-
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. In ovarian somatic cells, mediates silencing of transposable elements at the transcriptional level in a mael-dependent manner. Involved in silencing of long terminal repeat (LTR) retrotransposons in male germline. In testis, regulates spermatogenesis together with Tudor-SN. In germ cells, mediates silencing at both transcriptional and post-transcriptional levels and is involved in the maintenance of populations of primary and secondary piRNAs. Piwi-mediated transcriptional silencing is accompanied by the formation of His3 'Lys-9' trimethylated (H3K9me3) euchromatin and heterochromatin. In ovary, associates predominantly with antisense piRNAs that contain uridine at their 5' end. Association with sense piRNAs is also observed but to a lesser extent. Mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. Its role in stem cell maintenance does not seem to require nuclear localization. Required maternally for the posterior localization of osk and vas and for pole cell formation during oogenesis and early embryogenesis. Together with Hop and Hsp83, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation. Shows RNA cleavage activity, although is not required for any of its known functions. In the ovaries, forms a complex with nxf2, Panx and Nxt1 which acts as effectors of cotranscriptional transposon silencing.
  • 基因功能參考文獻：
    1. PAF1 knockdown enhances PIWI silencing of reporters when Piwi-interacting RNAs (piRNAs)-target the transcript region proximal to the promoter. Loss of PAF1 suppresses endogenous transposable element (TE) transcript maturation, whereas a subset of gene transcripts and long-non-coding RNAs adjacent to TE insertions are affected by PAF1 knockdown in a similar fashion to piRNA-targeted reporters. PMID: 28844648
    2. These data identify a role for Piwi in the regulation of somatic stem cell function. PMID: 28903034
    3. Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway. PMID: 28746365
    4. Report the mapping of multiple Piwi-interacting chromosomal domains in somatic cells of ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes. PMID: 28472469
    5. The identity of piRNA clusters is epigenetically acquired in a Piwi-dependent manner during embryonic development. PMID: 28457744
    6. Piwi-piRNA complexes regulate the interaction of chromatin components with target loci to maintain silencing of transposable elements through the modulation of chromatin accessibility. PMID: 27425411
    7. Analysis of piRNA clusters containing remnants of I-elements shows increased expression of the piRNA (PIWI-interacting RNA )precursors and enrichment by the Heterochromatin Protein 1 homolog, Rhino, in weak R strains, which is in accordance with stronger piRNA expression by these regions PMID: 28448516
    8. Piwi represses c-Fos in the somatic niche to regulate germ stem cell maintenance and differentiation and in the somatic follicle cells to affect morphogenesis. PMID: 27622269
    9. We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication. PMID: 26868596
    10. dFMRP cooperates with Piwi in maintaining genome integrity by silencing heterochromatic genes and suppressing transposon expression. PMID: 26842990
    11. We hypothesize that Piwi negatively regulates PRC2 binding by sequestering PRC2 in the nucleoplasm, thus reducing PRC2 binding to many targets and influencing transcription during oogenesis. PMID: 26780607
    12. Piwi nuclear localization in somatic cells is required for differentiation. Piwi regulates the somatic expression of Fasciclin 3 in gonadal development. PMID: 26119740
    13. Functions of piRNAs and the Piwi Protein in Drosophila PMID: 26087619
    14. These results implicate Piwi in RNA polymerase activity modulation and stress response in the nucleolus. We discuss possible noncanonical Piwi functions along with its canonical role in transposon silencing by piRNAs. PMID: 25916123
    15. This reporter platform will facilitate future dissections of the PIWI-targeting mechanism. PMID: 25336588
    16. The results lead us to propose that the interaction between Su(var)3-7 and piwi or aubergine controls important developmental processes independently of transposon silencing. PMID: 24820312
    17. Piwi is required in multiple cell types to control gerline stem cell lineage development in the Drosophila ovary. PMID: 24658126
    18. The results demonstrate that a significant increase in the H3K4me2 level upon nuclear Piwi loss is not accompanied by the alterations in H3K9me3 and HP1a levels for several germline-expressed transposons, suggesting that in this case Piwi prevents transcription by a mechanism distinct from H3K9 methylation. PMID: 24782529
    19. HP1a profiles in piwi mutant animals confirm that Piwi depletion leads to decreased HP1a levels in pericentric heterochromatin, particularly in transposable elements . PMID: 24068954
    20. Drosophila piwi mutants exhibit germline stem cell tumors that are sustained by elevated Dpp signaling.The direct requirement for Piwi for normal germline formation is largely restricted to larval stages. PMID: 23891114
    21. REVIEW: new findings reveal the Piwi-piRNA pathway as a more general mechanism of gene regulation PMID: 23465540
    22. Piwi plays multiple roles in the piRNA pathway, in part enforcing transposon repression through effects on local chromatin states and transcription but also participating in germ cell piRNA biogenesis PMID: 23392609
    23. Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID: 23392610
    24. in transgenic flies with the DDX catalytic triad of PIWI mutated, neither primary nor secondary piRNA biogenesis is detectably affected, transposons remain repressed, and fertility is normal PMID: 23297219
    25. Drosophila Piwi functions downstream of piRNA production mediating a chromatin-based transposon silencing mechanism in female germ line. PMID: 22160707
    26. Piwi function in germ-line stem cell (GSC) self-renewal is independent of transposon repression and is normally realized in the cytoplasm of GSC niche cells PMID: 22065765
    27. Piwi functions in Hsp90-mediated suppression of phenotypic variation PMID: 21186352
    28. results reveal a novel epigenetic mechanism involving Corto and Piwi that defines the fate and signaling function of niche cells in maintaining germline stem cells PMID: 20647505
    29. The impairing aubergine and piwi mutations also increase the level of germ-line mRNA from CP, a transgene that encodes the P-element transposase. PMID: 20943007
    30. Studies show that Drosophila melanogaster has three PIWI proteins: PIWI, AUBERGINE (AUB), and ARGONAUTE3 (AGO3) and it has been shown that Drosophila PIWI is mostly a nuclear protein [10] and is involved in the asymmetric division of germline stem cells. PMID: 19774126
    31. Mutations in piwi block posttranscriptional gene silencing of transgenic Adh. PMID: 11864605
    32. mutations in piwi, aubergine, or spindle-E which encode RNAi components result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2 PMID: 14752161
    33. Piwi maintains germline stem cells by repressing Bam expression, thus preventing Bam from downregulating Pum/Nos function in repressing the translation of differentiation genes and germline Piwi function in promoting germ cell division. PMID: 15668175
    34. Piwi maintains germline stem cells by silencing bam. PMID: 15668176
    35. PIWI protein is involved in silencing of LTR retrotransposons in testes. PMID: 15817569
    36. A genome-wide analysis of mRNAs regulated by piwi was performed. PMID: 16581772
    37. These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with repeat-associated small interfering RNAs originating from repetitive targets. PMID: 16882972
    38. Study examined piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and found that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. PMID: 17346786
    39. Pimet mediates piwi-interacting RNAs 2'-O-methylation in Drosophila. PMID: 17606638
    40. Piwi promotes euchromatic histone modifications and piRNA transcription in subtelomeric heterochromatin (also known as telomere-associated sequence, or TAS), on the right arm of chromosome 3 PMID: 17952056
    41. somatic silencing of two retrotransposons depends on Piwi in the follicle cells surrounding the oocyte. PMID: 18253480
    42. The Drosophila homologue of protein methyltransferase 5 (dPRMT5, csul/dart5)methylates arginine in Drosophila Piwi, Ago3 and Aub proteins in vivo. Arginine methylation of Piwi proteins is required for Ago3 and Aub stability. PMID: 19377467
    43. in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing PMID: 19812547

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  • 亞細胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Nucleus. Chromosome.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 組織特異性：
    Expressed in ovaries (at protein level). Expressed somatically in ovariole terminal filament cells, epithelial sheath cells, cap cells and follicle cells (at protein level). Expressed in nurse cells and oocytes in developing egg chambers (at protein level
  • 數據庫鏈接：