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  4. Recombinant Drosophila melanogaster Histone H2A.v (His2Av)

Recombinant Drosophila melanogaster Histone H2A.v (His2Av)

  • 中文名稱：
    Recombinant Drosophila melanogaster Histone H2A.v (His2Av)
  • 品名簡稱：
    Recombinant Drosophila melanogaster His2Av protein
  • 貨號：
    CSB-EP010095DLU
  • 說明書：
  • 規格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 基因名：
    His2Av
  • Uniprot No.：
  • 別名：
    H2A.F/Z
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    21.8 kDa
  • 表達區域：
    2-141aa
  • 氨基酸序列
    AGGKAGKDSGKAKAKAVSRSARAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKEETVQDPQRKGNVILSQAY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.113.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acts as a Polycomb group (PcG) protein required to maintain the transcriptionally repressive state of homeotic genes of the animal throughout development. Required for histone H3 'Lys-9' methylation and histone H4 'Lys-12' acetylation, two modifications that are essential for heterochromatin formation. Also involved in DNA double strand break (DSB) repair. Essential for early development.
  • 基因功能參考文獻：
    1. H2Av or H3S10 phosphorylation by JIL-1 is not required for chromatin decondensation or transcriptional elongation in Drosophila. PMID: 28807902
    2. The nucleosome positioning sequence (NPS) patterns from H2A.Z nucleosomes differ from the NPS patterns from H2A nucleosomes. The purine-purine/pyrimidine-pyrimidine pattern of H2A.Z nucleosomes has major peaks shifted by 10 bp deviated from the H2A nucleosome pattern. The H2A and H2A.Z nucleosomes have different sequence preferences. PMID: 27992255
    3. the H2AZ-like rather than the H2AX-like function of H2AV is primarily required for normal hematopoiesis. PMID: 28242611
    4. The Drosophila histone variant H2A.V works in concert with HP1 to promote kinetochore-driven microtubule formation. PMID: 25591068
    5. nucleosomes present significant, context-specific barriers to RNAPII in vivo that can be tuned by the incorporation of H2A.Z PMID: 24606920
    6. chromatin loosening and associated initiation of gene expression is activated by phosphorylation of H2Av in a nucleosome positioned in promoter regions of PARP-1-dependent genes PMID: 24508391
    7. Unlike other organisms that employ several H2A variants for different functions, the parsimonious fruit fly Drosophila melanogaster gets along with just a single H2A variant, H2A.V. Remarkably, H2A.V unites within one molecule features and functions of two different mammalian H2A variants, H2A.Z and H2A.X. [Review] PMID: 23553272
    8. H2A.Z nucleosome occupancy has no effect on sequence variability of Drosophila genome PMID: 23472174
    9. PARP1 is targeted to chromatin by association with the histone H2A variant (H2Av). PMID: 21444826
    10. in the absence of H2A.Z, checkpoint-defective telomeres adopt alternative structures, which are permissive for the loading of the capping machinery at Drosophila telomeres. PMID: 18845840

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  • 亞細胞定位：
    Nucleus. Chromosome. Note=Widely distributed in the genome, irrespective of the transcriptional status or coding capacity of the sequence.
  • 蛋白家族：
    Histone H2A family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG5499

    STRING: 7227.FBpp0084434

    UniGene: Dm.2346