Recombinant Caenorhabditis elegans Transcription factor elt-2 (elt-2)

中文名稱：

Recombinant Caenorhabditis elegans Transcription factor elt-2 (elt-2)
品名簡稱：

Recombinant Caenorhabditis elegans elt-2 protein
貨號：

CSB-EP607427CXY
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

elt-2
Uniprot No.：

Q10655
別名：

GATA-type domain-containing protein elt-2
種屬：

Caenorhabditis elegans
蛋白長度：

Full Length
來源：

E.coli
分子量：

54.0 kDa
表達(dá)區(qū)域：

1-433aa
氨基酸序列

MDNNYNDNVNGWAEMEPSQPMGGLRLPTQNMDPPEQNNESQLSELPRMKIDNDYASPIERQSVITSGTNNYEPKVETVTSFFHTGIDYSNFGMLDQTTMQPFYPLYSGIPVNTLGTFSGYTNSIYDKPSLYDPSIPTINIPSTYPTVAPTYECVKCSQSCGAGMKAVNGGMMCVNCSTPKTTYSPPVAYSTSLGQPPILEIPSEQPTAKIAKQSSKKSSSSNRGSNGSASRRQGLVCSNCNGTNTTLWRRNAEGDPVCNACGLYFKLHHIPRPTSMKKEGALQTRKRKSKSGDSSTPSTSRARERKFERASSSTEKAQRSSNRRAGSAKADRELSTAAVAAATATYVSHADLYPVSSAAVTLPDQTYSNYYQWNTAATAGLMMVPNDQNYVYAATNYQTGLRPADNIQVHVMPVQDDETKAAARDLEAVDGDS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Transcriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3'. Predominantly directs the transcription of intestinal genes such as ges-1, cpr-6, pho-1, ftn-1 and lev-11, and itself. Required for gut-specific differentiation, specifically acting with the GATA region-binding transcription factor elt-7 to control normal gene expression and promote normal formation of the intestine. Regulates intestinal gene expression in response to hypoxia to promote longevity. Regulates tissue specific gene expression at basal levels and in response to bacterial infection in the intestine to control innate immununity. Plays a role in the induction of metal-responsive genes, activating gene expression from zinc-activated promoters and iron-dependent promoters and enhancers. May regulate the expression of genes that control sensitivity to oxidative stress, in a mab-3-dependent manner, and osmotic stress, in conjunction with the GATA region-binding transcription factor elt-3. May play a role in sphingolipid signaling by regulating the expression of the sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase. May act with the Notch signaling pathway to promote endodermal gene expression. Has a protective role in response to infection by Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen C.neoformans. An association with the 26S proteasome regulatory subunit rpt-6, in part, controls gene expression in response to infection by P.aeruginosa. Regulates gene expression during the recovery phase following a bacterial infection. May act with p38-activated transcription factors to control p38 gene induction in response to bacterial infection. Controls lysosome formation in the intestine by controlling lysosomal gene expression.
基因功能參考文獻(xiàn)：
1. gene loss leads to modest increases in the level of ELT-2 protein in the early endoderm although ELT-2 levels do not strictly correlate with rescue. PMID: 29593072
2. Genes expressed only in the intestine showed 3 distinguishable classes of response to different mutant backgrounds. One class of genes responded as if ELT-2 is the major transcriptional activator and ELT-7 provides variable compensatory input. Appropriately expressed ELT-2 is able to replace all other core GATA factors in the C. elegans endoderm. PMID: 29360433
3. The authors determined that the GATA transcription factor ELT-2 and the p38 MAP kinase PMK-1 are necessary for animals to successfully recover from an acute Pseudomonas aeruginosa infection. PMID: 27600703
4. Overexpression of elt-2 extends lifespan and slows the rate of gene expression changes that occur during normal aging. Thus, our results identify the developmental regulator ELT-2 as a major driver of normal aging in C. elegans PMID: 27070429
5. GATA motifs played largely subtle roles in modulating postembryonic levels of elt-2 PMID: 26896592
6. ELT-2 is capable of specifying the entire C. elegans endoderm. PMID: 26700680
7. These results suggest that ELT-2 functions as a tissue-specific master regulator controlling the contribution of the p38 MAPK pathway to innate immune responses. PMID: 26016853
8. we found that recovery from an acute bacterial infection is dependent on ELT-2 activity. PMID: 25340560
9. An unexpected feature of the transcriptional response to Burkholderia pseudomallei was a progressive increase in the proportion of down-regulated genes, of which ELT-2 transcriptional targets were significantly enriched. PMID: 23980181
10. Intestinal distension is accelerated in elt-2 RNAi nematodes, and is observed in colonization but not toxin-based Pseudomonas infection. PMID: 21168435
11. the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, clec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection PMID: 17183709
12. Results identify a conserved function for endodermal GATA transcription factors GATA6 and ELT-2 in regulating local epithelial innate immune responses. PMID: 16968778
13. A simple model is proposed in which the ELT-2 GATA factor directly participates in the transcription of all intestine-specific/intestine-enriched genes, from the early embryo through to the dying adult. PMID: 17113066
14. Results suggest that ELT-2 plays a central role in most aspects of C. elegans intestinal physiology. PMID: 19111532
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亞細(xì)胞定位：

Nucleus.
組織特異性：

Expressed in the intestine.
數(shù)據(jù)庫鏈接：

KEGG: cel:CELE_C33D3.1

STRING: 6239.C33D3.1

UniGene: Cel.6404