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  4. Recombinant Bovine Transcription factor A, mitochondrial (TFAM)

Recombinant Bovine Transcription factor A, mitochondrial (TFAM)

  • 中文名稱：
    牛TFAM重組蛋白
  • 貨號：
    CSB-YP023413BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛TFAM重組蛋白
  • 貨號：
    CSB-EP023413BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛TFAM重組蛋白
  • 貨號：
    CSB-EP023413BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛TFAM重組蛋白
  • 貨號：
    CSB-BP023413BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛TFAM重組蛋白
  • 貨號：
    CSB-MP023413BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    TFAM; Transcription factor A; mitochondrial; mtTFA
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    43-246
  • 氨基酸序列
    SSSLSGYP KKPMTSYVRF SKEQLPIFKA QNPDAKNSEL IKKIAKLWRE LPDSEKKIYE DAYRADWQVY KEEINRIQEQ LTPSQMVSLE KEIMQKRLKK KALIKKRELT MLGKPKRPRS AYNIFIAERF QEARDGTSQV KLKAINENWK NLSNSQKQVY IQLAKDDKIR YYNEMKSWEE QMMEVGREDL IRRSIKYPAK NDPEKF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
  • 基因功能參考文獻：
    1. Our results suggest that TFAM plays an important role in lipid metabolism and may be a strong candidate gene for obesity in mammals. PMID: 16005429
    2. de novo mtTFA expression associated with mitochondrial biogenesis activation & high levels of nuclear respiratory factor-1 mRNA from oocyte stage onwards argue for essential function of these factors during the first steps of bovine embryogenesis. PMID: 16285882
  • 亞細胞定位：
    Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
  • 數據庫鏈接：


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