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  4. Recombinant Bovine Macrophage migration inhibitory factor (MIF)

Recombinant Bovine Macrophage migration inhibitory factor (MIF)

  • 中文名稱：
    牛MIF重組蛋白
  • 貨號：
    CSB-YP013826BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛MIF重組蛋白
  • 貨號：
    CSB-EP013826BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛MIF重組蛋白
  • 貨號：
    CSB-EP013826BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛MIF重組蛋白
  • 貨號：
    CSB-BP013826BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛MIF重組蛋白
  • 貨號：
    CSB-MP013826BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    MIF; Macrophage migration inhibitory factor; MIF; EC 5.3.2.1; L-dopachrome isomerase; L-dopachrome tautomerase; EC 5.3.3.12; Phenylpyruvate tautomerase; p12A
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-115
  • 氨基酸序列
    PMFVVNTNV PRASVPDGLL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLTERLRI SPDRIYINFC DMNAANVGWN GSTFA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 基因功能參考文獻：
    1. In conclusion, the bovine ampulla and isthmus have higher MIF expression during the postovulatory phase. PMID: 27465152
    2. plasma MIF concentrations may increase with age in months and parity, but do not change either before and after parturition or before and after postpartum first ovulation in Japanese black cows PMID: 26853787
    3. Data suggest that, in obese cows, expression of MIF is suppressed in the ampulla and isthmus of Fallopian tubes as compared to normal-weight cows; however, MIF expression is also lower in Fallopian tubes of lean cows. The primary site of MIF expression in Fallopian tube ampulla/isthmus is the tunica mucosa. These studies were conducted in Japanese Black calves. PMID: 25426961
    4. The objective of the present study was to determine if SNPs in 5' region of bovine MIF affects its promoter activity. PMID: 21774622
    5. MIF plays a role in early embryo development, and further characterization of MIF expression and its regulation in the endometrium will add significantly to our understanding of early embryo-uterine interactions PMID: 12855605
    6. The diverse actions of MIF within the immuno-neuroendocrine system may be a result of its occurrence in different isoforms and oligomerization states. PMID: 15756943
    7. The purification of macrophage migration inhibitory factor (MIF) from bovine brain cytosol and its partial characterization are reported. PMID: 16457622

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  • 亞細胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    MIF family
  • 數據庫鏈接：


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