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  4. Recombinant Arabidopsis thaliana Protein phosphatase 2C 37 (PP2CA)

Recombinant Arabidopsis thaliana Protein phosphatase 2C 37 (PP2CA)

  • 中文名稱：
    擬南芥PP2CA重組蛋白
  • 貨號：
    CSB-YP342241DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PP2CA重組蛋白
  • 貨號：
    CSB-EP342241DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PP2CA重組蛋白
  • 貨號：
    CSB-EP342241DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PP2CA重組蛋白
  • 貨號：
    CSB-BP342241DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PP2CA重組蛋白
  • 貨號：
    CSB-MP342241DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PP2CA
  • Uniprot No.：
  • 別名：
    PP2CA; AHG3; At3g11410; F24K9.8Protein phosphatase 2C 37; AtPP2C37; EC 3.1.3.16; Protein ABA-HYPERSENSITIVE GERMINATION 3; Protein phosphatase 2C A; PP2CA
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-399
  • 氨基酸序列
    MAGICCGVVG ETEPAAPVDS TSRASLRRRL DLLPSIKIVA DSAVAPPLEN CRKRQKRETV VLSTLPGNLD LDSNVRSENK KARSAVTNSN SVTEAESFFS DVPKIGTTSV CGRRRDMEDA VSIHPSFLQR NSENHHFYGV FDGHGCSHVA EKCRERLHDI VKKEVEVMAS DEWTETMVKS FQKMDKEVSQ RECNLVVNGA TRSMKNSCRC ELQSPQCDAV GSTAVVSVVT PEKIIVSNCG DSRAVLCRNG VAIPLSVDHK PDRPDELIRI QQAGGRVIYW DGARVLGVLA MSRAIGDNYL KPYVIPDPEV TVTDRTDEDE CLILASDGLW DVVPNETACG VARMCLRGAG AGDDSDAAHN ACSDAALLLT KLALARQSSD NVSVVVVDLR KRRNNQASS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Major negative regulator of abscisic acid (ABA) responses during seed germination and cold acclimation. Confers insensitivity to ABA. Modulates negatively the AKT2/3 activity, which mediates K(+) transport and membrane polarization during stress situations, probably by dephosphorylation. Prevents stomata closure by inactivating the S-type anion efflux channel SLAC1 and its activator SRK2E. Represses KIN10 activity by the specific dephosphorylation of its T-loop Thr-198, leading to a poststress inactivation of SnRK1 signaling.
  • 基因功能參考文獻：
    1. The interaction of AtPP2CA with "phosphorylated" or "dephosphorylated" GORK systematically led to inhibition of the channel to the same baseline level. PMID: 26801610
    2. Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm. PMID: 26466609
    3. ABI1 and PP2CA dephosphorylate and inactivate SnRK1 which represses SnRK1 signaling. PMID: 24179127
    4. The structure of PP2CA revealed an uncharacterized CCCH zinc-finger motif. PMID: 24165892
    5. Data indicate that PYL8 interacted in vivo with at least five PP2Cs, namely HAB1, HAB2, ABI1, ABI2, and PP2CA. PMID: 23370718
    6. ABA-hypersensitive germination3 (AHG3)encodes AtPP2CA that functions as a negative regulator of the ABA-signaling pathway and plays a major role in ABA signaling in seed germination and early growth. [AHG3] PMID: 16339800
    7. The AtPP2CA protein phosphatase is a strong negative regulator of ABA signal transduction. [AtPP2CA] PMID: 16361522

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  • 蛋白家族：
    PP2C family
  • 組織特異性：
    Mostly expressed in seeds and leaves, and, to a lower extent, in roots, stems, and flowers, particularly in siliques. Essentially found in the phloem.
  • 數據庫鏈接：

    KEGG: ath:AT3G11410

    STRING: 3702.AT3G11410.1

    UniGene: At.20739