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  4. Recombinant Arabidopsis thaliana Auxin-responsive protein IAA3 (IAA3)

Recombinant Arabidopsis thaliana Auxin-responsive protein IAA3 (IAA3)

  • 中文名稱：
    Recombinant Arabidopsis thaliana Auxin-responsive protein IAA3 (IAA3)
  • 品名簡稱：
    Recombinant Mouse-ear cress IAA3 protein
  • 貨號：
    CSB-EP653623DOA
  • 說明書：
  • 規格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    IAA3
  • Uniprot No.：
  • 別名：
    Indoleacetic acid-induced protein 3;Short hypocotyl;Suppressor of HY2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    28.4 kDa
  • 表達區域：
    1-189aa
  • 氨基酸序列
    MDEFVNLKETELRLGLPGTDNVCEAKERVSCCNNNNKRVLSTDTEKEIESSSRKTETSPPRKAQIVGWPPVRSYRKNNIQSKKNESEHEGQGIYVKVSMDGAPYLRKIDLSCYKGYSELLKALEVMFKFSVGEYFERDGYKGSDFVPTYEDKDGDWMLIGDVPWEMFICTCKRLRIMKGSEAKGLGCGV
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Plays a central role in auxin regulation of root growth, in gravitropism, and in lateral root formation. Regulated by an auxin-induced protein turnover. Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. When activated by cytokinin, restricts the expression of the PIN genes to the vascular transition zone. Induction of SHY2 in the vascular transition zone restricts BRX expression to down-regulate PIN3 and thus limit meristem growth, but proper SHY2 expression requires BRX. Involved in meristem growth and in determining its size. May participate in strigolactone signaling to regulate meristem size and lateral root formation.
  • 基因功能參考文獻：
    1. SHY2 is a pivotal negative regulator of root growth, which has been demonstrated to efficiently repress transcription of major auxin transport facilitators of the PIN-FORMED (PIN) gene family, thereby restricting polar auxin transport to the root tip and in consequence auxin-driven primary root growth. Taken together, our results disclose the central low-energy activated SnRK1-C/S1-bZIP signalling module as gateway to PMID: 28158182
    2. DA1-related protein 2 (DAR2) acts downstream of cytokinin and SHY2 but upstream of PLT1/2 to affect root meristem size. PMID: 23518585
    3. Strigolactone signaling involves SHY2 protein activity in roots. PMID: 23425316
    4. The shy2 mutations strongly elevate endogenous indole-3-acetic acid level in the roots. PMID: 22527388
    5. Data suggest a model in which the regulatory interactions favor BRX expression in the early proximal meristem and SHY2 prevails because of supplementary cytokinin induction in the later distal meristem. PMID: 21149702

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Aux/IAA family
  • 組織特異性：
    Highly expressed in stems and flowers. Expressed in hypocotyls, cotyledons and leaves, but barely detected in roots. Expressed in root tips. In the root meristem, specifically detected at the vascular tissue transition zone.
  • 數據庫鏈接：

    KEGG: ath:AT1G04240

    STRING: 3702.AT1G04240.1

    UniGene: At.20801