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  4. TRMT2A Recombinant Monoclonal Antibody

TRMT2A Recombinant Monoclonal Antibody

  • 中文名稱：
    TRMT2A Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA223383A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      , Positive WB detected in:MCF7 whole cell lysate(30μg), Hela whole cell lysate(30μg)
      All lanes: TRMT2A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 69 kDa
      Observed band size: 75 kDa
      Exposure time：60s
    • IHC image of CSB-RA223383A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA223383A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HeLa cell with CSB-RA223383A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    TRMT2A
  • 別名：
    HpaII tiny fragments locus 9c protein antibody; HTF9C antibody; TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody; TRM2 tRNA methyltransferase 2 homolog A antibody; TRM2A_HUMAN antibody; TRMT2A antibody; tRNA (uracil-5-)-methyltransferase homolog A antibody; tRNA methyltransferase 2 homolog A antibody; tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide from human TRMT2A protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5B1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    May be involved in nucleic acid metabolism and/or modifications.
  • 基因功能參考文獻(xiàn)：
    1. TRMT2A staining showed a strong correlation with likelihood of recurrence at five years in 67 HER2+ breast cancer. PMID: 20307320
    2. Single nucleotid polymorphisms of this gene may be associated with a deficit in sustained attention within schizophrenia, in a Taiwanese cohort. PMID: 18075473
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, RNA M5U methyltransferase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 24974

    OMIM: 611151

    KEGG: hsa:27037

    STRING: 9606.ENSP00000252136

    UniGene: Hs.713579