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TNPO3 Recombinant Monoclonal Antibody

  • 中文名稱(chēng)：
    TNPO3 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA081319A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of transportin 3 expression in HepG2 cells using transportin 3 antibody. Green, isotype control; red, transportin 3.
    • Immunocytochemical staining of HepG2 cells with transportin 3 antibody. Nuclei were stained blue with DAPI; Transportin 3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
    • Western blotting analysis using transportin 3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with transportin 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    TNPO3
  • 別名：
    TNPO3; Transportin 3; TRN-SR; IPO12; TRN-SR2; MTR10A; Limb Girdle Muscular Dystrophy 1F (Autosomal Dominant); Transportin-SR; Transportin-3; Importin 12; LGMD1F; Imp12; Importin-12; LGMDD2; TRNSR
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human TNPO3 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    6C2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Importin, which transports target proteins into the nucleus. Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains. Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation. The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively. Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus.; (Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus. Required for a nuclear maturation step of HIV-1 prior to integration.
  • 基因功能參考文獻(xiàn)：
    1. Combining these results with small-angle X-ray scattering data for the complex of TRN-SR2 with truncated integrase, we propose a molecular model of the complex. We speculate that nuclear import of the PIC may proceed concurrently with the normal nuclear transport. PMID: 28356354
    2. IRF5-TNPO3 genetic variation is associated systemic lupus erythematosus. PMID: 25205108
    3. Data suggest that the HIV-1 integrase (IN)/transportin-SR2 (TRN-SR2) interaction interface is a potential target for antiviral therapy. PMID: 25063804
    4. Tnpo3 mutants that are not able to interact with cleavage and polyadenylation specificity factor 6 do not facilitate HIV-1 infectivity, suggesting a potential route of pharmacological intervention in the treatment of AIDS. PMID: 24449914
    5. TNPO3 mutation is the cause of limb-girdle muscular dystrophy 1F, expands knowledge of the molecular basis of muscular dystrophies and bolsters the importance of defects of nuclear envelope proteins as causes of inherited myopathies. PMID: 23543484
    6. The TNPO3 gene is mapped within the Limb-girdle muscular dystrophy 1F critical interval and its 923-amino acid human gene product is also expressed in skeletal muscle. PMID: 23667635
    7. a model wherein one monomer of TRN-SR2 is bound to one monomer of RanGTP. PMID: 23878195
    8. In skeletal muscle of limb-girdle muscular dystrophy 1F individuals, expression of transportin 3 indicates altered transportin 3 function. PMID: 23543484
    9. These results suggest that TNPO3 and cyclophilin A facilitate HIV-1 infection by coordinating proper uncoating of the core in target cells. PMID: 23097435
    10. Identification of residues in the C-terminal domain of HIV-1 integrase that mediate binding to the transportin-SR2 protein. PMID: 22872638
    11. TNPO3 can directly engage the HIV-1 IN tetramer prebound to the cognate DNA. PMID: 22872640
    12. Transportin 3 and importin alpha act as receptors and are required for effective nuclear import of HIV-1 integrase in virus-infected cells. PMID: 21326825
    13. TNPO3 interacts with HIV-1 gag in the cytoplasm to assist HIV-1 infection after nuclear import. PMID: 22398280
    14. TNPO3 promotes HIV-1 infectivity at a step in the virus life cycle that is detectable after the preintegration complex arrives in the nucleus and capsid is the viral determinant for TNPO3 dependence. PMID: 22145813
    15. TNPO3 binds to a surface of monomeric HIV-1 integrase that remains exposed after tetramerization. PMID: 22176773
    16. a novel nuclear localization signal and mechanism for serpinF1 nuclear import PMID: 22028839
    17. The authors demonstrated that TNPO3 was required by several lentiviruses for nuclear import. PMID: 21976643
    18. Tnp3 binds to tRNAs and capsids proteins, and is required for efficient HIV-1 integration. PMID: 21901095
    19. Here the authors discover a link between the viral entry of HIV and its interaction with TRN-SR2. PMID: 21276267
    20. capsid, not integrase, is the dominant viral factor that dictates transportin 3 dependency during HIV-1 infection PMID: 19846519
    21. Results imply that the mechanism by which SR proteins are imported to the nucleus is conserved between Drosophila and humans and involves the nuclear import receptor transportin-SR PMID: 12134081

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  • 相關(guān)疾?。?/div>
    Limb-girdle muscular dystrophy 1F (LGMD1F)
  • 亞細(xì)胞定位：
    Nucleus envelope. Cytoplasm.
  • 組織特異性：
    Expressed in skeletal muscle.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17103

    OMIM: 608423

    KEGG: hsa:23534

    STRING: 9606.ENSP00000265388

    UniGene: Hs.193613