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  4. SNX1 Recombinant Monoclonal Antibody

SNX1 Recombinant Monoclonal Antibody

  • 中文名稱：
    SNX1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA986243A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HT-29 whole cell lysate(30μg), A431 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HL-60 whole cell lysate(30μg), HEK293T whole cell lysate(30μg)
      All lanes: Sorting nexin 1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 59 kDa
      Observed band size: 74 kDa
      Exposure time：2min
    • Immunofluorescence staining of Hela cell with CSB-RA986243A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA986243A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Sorting nexin-1, SNX1
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human SNX1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8E11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking.
  • 基因功能參考文獻：
    1. Data suggest that GLP1R signaling in pancreatic beta-cells leading to insulin secretion involves interactions of GLP1R with HIP1, SNX1, and SNX27; HIP1 appears to regulate coupling of cell surface GLP1R activation with endocytosis; SNX1 and SNX27 appear to control balance between GLP1R plasma membrane recycling and lysosomal degradation. PMID: 29284659
    2. SNX1 was down-regulated in CRC and associated with poor prognosis. Our data also showed that SNX1 inhibited the proliferation of CRC cells and increased the sensitivity to the most commonly used drugs for CRC. PMID: 26643894
    3. miR-95 functions as an oncogene role in non-small cell lung cancer cells by directly targeting SNX1. PMID: 24835695
    4. Results show that suppression of EGFR membrane recycling by SNX1 appears to be critical for the activation of EGFR /PI3K/AKT signaling pathway in human lung cancer cells. PMID: 25653196
    5. we suggest that SNX1 is involved in the negative regulation of ligand-induced EGFR phosphorylation and mediates EGFR/pEGFR trafficking out of early endosomes PMID: 22859339
    6. SNX1 has a crucial role in D(5)R trafficking and SNX1 depletion results in D(5)R dysfunction and thus may represent a novel mechanism for the pathogenesis of essential hypertension PMID: 23152498
    7. This study found that significant evidence of association with SNX1 (VEGAS p = 0.035) in patient with Alzheimer disease. PMID: 22673115
    8. SNX4, but not SNX1 and SNX8, is associated with the Rab11-recycling endosomes and that a high frequency of SNX4-mediated tubule formation is observed as endosomes undergo Rab4-to-Rab11 transition. PMID: 21973056
    9. Results demonstrate that miR-95 increases proliferation by directly targeting SNX1, defining miR-95 as a new oncogenic miRNA in CRC. PMID: 21427358
    10. SNX1 and SNX2 interact with Kalirin-7. Overexpression of SNX1 or SNX2 and Kalirin-7 partially redistributes both SNXs to the plasma membrane, and results in RhoG-dependent lamellipodia formation. PMID: 20604901
    11. These data describe a novel function of SNX1 in the regulation of P2Y(1) receptor recycling and suggest that SNX1 plays multiple roles in endocytic trafficking of G-protein coupled receptors. PMID: 20070609
    12. controls down-regulation of protease-activated receptor-1 PMID: 12058063
    13. PX domain-dependent/early endosomal association of SNX1 is important for its ability to regulate the targeting of internalized epidermal growth factor receptor for lysosomal degradation PMID: 12198132
    14. identified sorting nexin 1 (SNX1) as a novel partner of enterothelin-1 (ENT-1); show that Ent-1 and SNX1 co-eluted in macromolecular complexes containing part of epidermal growth factor receptor(EGFR) PMID: 12657642
    15. SNX1 associates with a microdomain of the early endosome where it regulates tubular-based endosome-to-trans Golgi network retrieval of the cation-independent mannose-6-phosphate receptor. PMID: 15498486
    16. NMR structure of the PX domain of SNX1 reveals an overall fold that is similar to high-affinity PX domains PMID: 15673616
    17. HkRP1 is involved in the process of tubulation of sorting nexin-1 positive membranes from early endosome subdomains PMID: 15882442
    18. These findings establish an essential role for endogenous SNX1 in sorting activated PAR1 to a distinct lysosomal degradative pathway that is independent of retromer, Hrs, and Tsg101. PMID: 16407403
    19. Overexpression of SNX1 is able to attenuate the effect of SNX5 on epidermal growth factor (EGF) receptor degradation. PMID: 16487940
    20. These observations indicate that the mammalian retromer complex assembles by sequential association of SNX1/2 and Vps26-Vps29-Vps35 subcomplexes on endosomal membranes and that SNX1 and SNX2 play interchangeable but essential roles. PMID: 17101778
    21. The loss of SNX1 may play a significant role in the development and aggressiveness of human colon cancer, at least partially through the mechanism of increased signaling from endosomes. PMID: 17145813
    22. Together, these findings demonstrate a role for SNX1 in retrieval of E-cadherin from a degradative endosomal pathway and in membrane trafficking pathways that regulate E-cadherin recycling. PMID: 17502486
    23. sortilin and mannose-6-phosphate receptors recycle to the TGN in SNX1-dependent carriers, which we named endosome-to-TGN transport carriers PMID: 18088323

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  • 亞細胞定位：
    Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium.
  • 蛋白家族：
    Sorting nexin family
  • 數據庫鏈接：

    HGNC: 11172

    OMIM: 601272

    KEGG: hsa:6642

    STRING: 9606.ENSP00000453785

    UniGene: Hs.188634



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