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  4. RAVER2 Recombinant Monoclonal Antibody

RAVER2 Recombinant Monoclonal Antibody

  • 中文名稱：
    RAVER2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA299895A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), 780-O whole cell lysate(30μg), A549 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), Raw264.7 whole cell lysate(30μg)
      All lanes: RAVER2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 74 kDa
      Observed band size: 85 kDa
      Exposure time：1min
    • Immunofluorescence staining of SH-SY5Y cell with CSB-RA299895A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA299895A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    RAVER2
  • 別名：
    Ribonucleoprotein PTB-binding 2 (Protein raver-2), RAVER2, KIAA1579
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Antibody
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4B7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    May bind single-stranded nucleic acids.
  • 基因功能參考文獻：
    1. Deregulation of LMNB1 expression induces modified splicing of several genes, likely driven by raver-2 overexpression, and suggest that an alteration of mRNA processing could be a pathogenic mechanism in adult-onset autosomal dominant leukodystrophy. PMID: 25637521
    2. genetic polymorphism is ssociated with increased susceptibility for ulcerative colitis PMID: 22561236
    3. Together, our results suggest that regulation of human Raver2 expression involves TATA-less transcriptional activity. PMID: 22146317
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 數據庫鏈接：

    HGNC: 25577

    OMIM: 609953

    KEGG: hsa:55225

    STRING: 9606.ENSP00000360112

    UniGene: Hs.591443