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  4. PTBP2 Recombinant Monoclonal Antibody

PTBP2 Recombinant Monoclonal Antibody

  • 中文名稱：
    PTBP2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA201933A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      , Positive WB detected in: SH-SY5Y whole cell lysate(30μg), U251 whole cell lysate(30μg), LO2 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
      All lanes: PTBP2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 57 kDa
      Observed band size: 57 kDa
      Exposure time：60s
    • IHC image of CSB-RA201933A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA201933A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of SH-SY5Y cell with CSB-RA201933A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA201933A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    brPTB antibody; FLJ34897 antibody; MIBP antibody; Neural polypyrimidine tract binding protein antibody; Neural polypyrimidine tract-binding protein antibody; Neurally enriched homolog of PTB antibody; Neurally-enriched homolog of PTB antibody; nPTB 5 antibody; nPTB 6 antibody; nPTB 7 antibody; nPTB 8 antibody; nPTB antibody; nPTB5 antibody; nPTB6 antibody; nPTB7 antibody; nPTB8 antibody; Polypyrimidine tract binding protein 2 antibody; Polypyrimidine tract-binding protein 2 antibody; PTB antibody; PTB like antibody; PTB like protein antibody; PTB-like protein antibody; PTBLP antibody; PTBP 2 antibody; Ptbp2 antibody; PTBP2_HUMAN antibody; Splicing regulator antibody
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide from human PTBP2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    3E1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.; Reduced affinity for RNA.
  • 基因功能參考文獻：
    1. It has been established that PTBP1 and PTBP2 are members of a family of cryptic exon repressors. PMID: 27681424
    2. PTBP1 and PTBP2 impaired autoregulation of SRSF3 in oral squamous cell carcinoma cancer cells. PMID: 26416554
    3. Data show that polypyrimidine tract-binding proteins nPTB and ROD1 interact with mitochondrial tRNA(Thr) in the cytoplasm outside of mitochondria. PMID: 26657638
    4. In T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1. PMID: 24286314
    5. MALAT1 binds to SFPQ releasing PTBP2 from the SFPQ/PTBP2 complex, the increased SFPQ-detached PTBP2 promotes cell proliferation and migration in colorectal cancer. PMID: 25025966
    6. Defining the multifunctional roles of PTB will contribute to the understanding of key regulatory events in gene expression. PMID: 24264039
    7. Changes in miR-223/PTBP2 pathway could contribute of abnormal splicing in chronic myeloid leukemia. PMID: 23174904
    8. Regulation of the mutually exclusive exons 8a and 8 in the CaV1.2 calcium channel transcript by polypyrimidine tract-binding protein. PMID: 21282112
    9. present fluorescence, NMR, and in vivo splicing data in support of a role of PTB in inducing RNA loops. We show that the RNA recognition motifs (RRMs) 3 and 4 of PTB can bind two distant pyrimidine tracts and bring their 5' and 3' ends in close proximity PMID: 20160105
    10. The nPTB proximal promoter, although rich in G+C content and presenting putative binding sites for the transcription factors Sp1, NF-1, NF-kB and Oct-1, lacks a typical TATA box. PMID: 16002244
    11. Study shows that PTB can function as an anti-repressor molecule to counteract the splicing inhibitory activity of SRp30c. PMID: 17548433

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  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform
  • 數據庫鏈接：

    HGNC: 17662

    OMIM: 608449

    KEGG: hsa:58155

    STRING: 9606.ENSP00000412788

    UniGene: Hs.596061