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  4. PPCS Recombinant Monoclonal Antibody

PPCS Recombinant Monoclonal Antibody

  • 中文名稱：
    PPCS Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA790244A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HEK293 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), A431 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
      All lanes: PPCS antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 34 kDa
      Observed band size: 34 kDa
      Exposure time：10s
    • IHC image of CSB-RA790244A0HU diluted at 1:100 and staining in paraffin-embedded mouse kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    PPCS
  • 別名：
    COAB antibody; FLJ11838 antibody; MGC117357 antibody; MGC138220 antibody; OTTHUMP00000008433 antibody; Phosphopantothenate--cysteine ligase antibody; Phosphopantothenoylcysteine synthetase antibody; PPC synthetase antibody; Ppcs antibody; PPCS_HUMAN antibody; RP11-163G10.1 antibody
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide from human PPCS protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    6D12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. Has a preference for ATP over CTP as a cosubstrate.
  • 基因功能參考文獻：
    1. Exome sequencing in five individuals from two unrelated families presenting with dilated cardiomyopathy revealed biallelic mutations in PPCS. PMID: 29754768
    2. Oxygen transfer studies found that 18O from [carboxyl-18O] phosphopantothenate is incorporated into AMP or CMP produced during PPCS catalysis, consistent with the formation of a phosphopantothenoyl cytidylate or phosphopantothenoyl adenylate intermediate PMID: 19683078
  • 蛋白家族：
    PPC synthetase family
  • 數據庫鏈接：

    HGNC: 25686

    OMIM: 609853

    KEGG: hsa:79717

    STRING: 9606.ENSP00000361642

    UniGene: Hs.706662