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NUP50 Recombinant Monoclonal Antibody

  • 中文名稱：
    NUP50 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA588009A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HL-60 whole cell lysate(30μg)
      All lanes: NUP50 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 50 kDa
      Observed band size: 58 kDa
      Exposure time：2min
    • IHC image of CSB-RA588009A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing A431 cells stained with CSB-RA588009A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    NUP50
  • 別名：
    50 kDa nucleoporin antibody; NPAP60 antibody; NPAP60L antibody; Nuclear pore associated protein 60L antibody; Nuclear pore complex protein Nup50 antibody; Nuclear pore-associated protein 60 kDa-like antibody; Nucleoporin 50 antibody; Nucleoporin 50kDa antibody; Nucleoporin Nup50 antibody; Nup50 antibody; NUP50_HUMAN antibody; PRO1146 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide from human NUP50 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    15A12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
  • 基因功能參考文獻：
    1. Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin alpha5. PMID: 22130666
    2. Results suggest that Npap60L and Npap60S function differently in nuclear protein import. PMID: 20016008
  • 亞細胞定位：
    Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.
  • 組織特異性：
    Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 8065

    OMIM: 604646

    KEGG: hsa:10762

    STRING: 9606.ENSP00000345895

    UniGene: Hs.475103