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  4. MDH1 Recombinant Monoclonal Antibody

MDH1 Recombinant Monoclonal Antibody

  • 中文名稱：
    MDH1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA280048A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      , Positive WB detected in:Jurkat whole cell lysate(30μg), HepG2 whole cell lysate(30μg), U251 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), PC-3 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Rat heart lysate(30μg)
      All lanes: MDH1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
      Exposure time：5s
    • IHC image of CSB-RA280048A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing A431 cells stained with CSB-RA280048A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    MDH1
  • 別名：
    cytoplasmic antibody; Cytosolic malate dehydrogenase antibody; Diiodophenylpyruvate reductase antibody; Malate dehydrogenase 1, NAD (soluble) antibody; Malate dehydrogenase antibody; Malate dehydrogenase cytoplasmic antibody; MDH s antibody; mdh1 antibody; MDHA antibody; MDHC_HUMAN antibody; MDHs antibody; MGC:1375 antibody; MOR2 antibody; Soluble malate dehydrogenase antibody
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide from human MDH1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8D3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.
  • 基因功能參考文獻：
    1. expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in non-small cell lung carcinoma cell lines. PMID: 29574159
    2. Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific. PMID: 27881739
    3. Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD. PMID: 28263970
    4. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
    5. adipogenic differentiation may be regulated by the acetylation of MDH1 PMID: 22693256
    6. The MDH1 gene is not the cause of RP28-linked autosomal recessive retinitis pigmentosa. PMID: 20011630
    7. expression of MDH1 is maintained in the adult heart but is not present in levels as high as in the fetus PMID: 15565635
    8. Malate Dehydrogenase directly regulates the Tumor Suppressor Protein p53-dependent apoptosis upon glucose deprivation and involved in maintaining cellular metabolic state and further determining cell death. PMID: 19229245

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    LDH/MDH superfamily, MDH type 2 family
  • 數據庫鏈接：

    HGNC: 6970

    OMIM: 154200

    KEGG: hsa:4190

    STRING: 9606.ENSP00000438144

    UniGene: Hs.526521



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