Western Blot
Positive WB detected in: Hela whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HepG2 whole cell lysate(30μg), SW620 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Mouse brain tissue lysate(30μg)
All lanes: MEK1/2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43, , 44 kDa
Observed band size: 50 kDa
Exposure time：40s

Immunofluorescence staining of A431 cell with CSB-RA788487A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA788487A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.