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  4. MAD2L1BP Recombinant Monoclonal Antibody

MAD2L1BP Recombinant Monoclonal Antibody

  • 中文名稱：
    MAD2L1BP Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA155738A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA155738A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA155738A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA155738A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing A431 cells stained with CSB-RA155738A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    MAD2L1BP
  • 別名：
    Caught by MAD2 protein antibody; CMT2 antibody; dJ261G23.1 antibody; KIAA0110 antibody; MAD2L1 binding protein antibody; MAD2L1 binding protein isoform 2 antibody; MAD2L1-binding protein antibody; Mad2l1bp antibody; MD2BP_HUMAN antibody; MGC11282 antibody; OTTHUMP00000016496 antibody; RP1 261G23.6 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human MAD2L1BP protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    18A12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
  • 基因功能參考文獻：
    1. MAD2-p31(comet) axis may serve as a potential therapeutic target for glioma. PMID: 29408509
    2. p31comet-induced cell death is mediated by interactions with Mad2 PMID: 26544187
    3. joint action of TRIP13 and p31(comet) also promotes the release of Mad2 from MCC, participates in the complete disassembly of MCC and abrogates checkpoint inhibition of APC/C PMID: 25092294
    4. Altered p31(Comet):Mad2 expression ratios may provide new insight into altered spindle assembly checkpoint function and the generation of chromosomal instability in tumors. PMID: 24131926
    5. attenuating the affinity of p31(Comet) for Mad2 by phosphorylation promotes SAC activity in mitosis. Specifically, phosphorylation of Ser-102 weakens p31(Comet)-Mad2 binding and enhances p31(Comet)-mediated bypass of the SAC. PMID: 24596092
    6. study found p31(comet) traffics on and off kinetochores and also present in the cytosol; Cells depleted of p31(comet) arrest in metaphase with mature bipolar kinetochore-microtubule attachments; propose that p31(comet) is an essential component of machinery that silences the checkpoint during each cell cycle PMID: 21965286
    7. the importance of p31(comet) in checkpoint silencing and its potential as a target for antimitotic therapies. PMID: 22544748
    8. The binding of p31(comet) to Mad2 in mitotic checkpoint complex may trigger a conformational change in Cdc20 that facilitates its phosphorylation by Cdk, and that the latter process may promote its dissociation from BubR1. PMID: 22566641
    9. both p31(comet) and ubiquitination of Cdc20 are critical mechanisms of checkpoint inactivation. They act redundantly to promote Mad2 dissociation from Cdc20 PMID: 21937719
    10. p31(comet) negatively regulates the spindle assembly checkpoint by extracting Mad2 from the MCC. PMID: 22100920
    11. This review presents knowledge of CMT2 and possible pathogenetic mechanisms responsible for the disease PMID: 22235654
    12. p31comet localizes to kinetochores in a C-Mad2-dependent manner. the Mad1:C-Mad2 complex undergoes regulation by p31comet-dependent 'capping'. PMID: 21772247
    13. Although p31(comet) binds to Mad2, it promotes the dissociation of Cdc20 from BubR1 in MCC. PMID: 21300909
    14. p31(comet, formerly known as Cmt2) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint PMID: 15257285
    15. p31(comet) does not have an impact on the formation of aneuploidy and tetraploidy found in human hepatocellular carcinoma. PMID: 17934339
    16. Data suggest that p31(comet)(CMT2) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2." PMID: 18022368
    17. p31(comet) induces tumor cell senescence by mediating p21(Waf1/Cip1) accumulation and Mad2 disruption and that these effects are dependent on a direct interaction of p31(comet) with Mad2. PMID: 19276188

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  • 亞細胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Note=During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
  • 蛋白家族：
    MAD2L1BP family
  • 數據庫鏈接：

    HGNC: 21059

    KEGG: hsa:9587

    STRING: 9606.ENSP00000410818

    UniGene: Hs.122346



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