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  4. KIFC1 Recombinant Monoclonal Antibody

KIFC1 Recombinant Monoclonal Antibody

  • 中文名稱：
    KIFC1 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA057545A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg)
      All lanes: KIFC1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 74 kDa
      Observed band size: 74 kDa
      Exposure time：3min
    • IHC image of CSB-RA057545A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing jurkat cells stained with CSB-RA057545A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    KIFC1
  • 別名：
    Kinesin-like protein KIFC1 (Kinesin-like protein 2) (Kinesin-related protein HSET), KIFC1, HSET KNSL2
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Antibody
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    1F9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Regulates cilium formation and structure.
  • 基因功能參考文獻(xiàn)：
    1. Data show that kinesin-related protein HSET (HSET) is incapable of forming asters from preformed, nongrowing microtubule (non-MT) MTs, but rapidly forms MT asters in the presence of soluble (non-MT) tubulin. PMID: 29985404
    2. KIFC1 interacts with F508del-CFTR. PMID: 30066085
    3. nuclear KIFC1 (nKIFC1) correlated significantly with Ki67 in White triple-negative breast cancer (TNBCs) but not in African American (AA) TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. PMID: 28218233
    4. Results identified Stathmin as a direct target of KIFC1. PMID: 28885720
    5. The overexpression of KIFC1 was correlated closely with the progression of hepatocellular carcinoma (HCC) and poor prognosis, and suggested that the expression levels of KIFC1 are a potential prognostic biomarker for HCC. PMID: 29620256
    6. Circulating pri-miRNA-944 and 3662 can improve non-invasive potential non-small cell lung cancer detection of operable stages of SCC and AC PMID: 28964573
    7. These findings reveal a new stationary binding model that kinesin-14 KIFC1 proteins function as crosslinkers between the Golgi apparatus and the microtubules and contribute to the central positioning and structural maintenance of the Golgi apparatus. PMID: 28430595
    8. Taken together, these results suggest that up-regulation of KIFC1 may induce docetaxel resistance. PMID: 27665358
    9. Low post translational modifications of KIFC1 protein is associated with neoplasms. PMID: 28209915
    10. KIFC1 is overexpressed in GC. PMID: 27176706
    11. Association of KIFC1 gene expression and centrosome amplification with high-grade serous ovarian carcinoma. PMID: 26992853
    12. stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could promote survival of cancer cells containing supernumerary centrosomes. PMID: 26987684
    13. KIFC1 silencing significantly reduces breast cancer cell viability. PMID: 26177331
    14. Data provides the first evidence of centrosome clustering-independent activities of HSET that fuel tumor progression. PMID: 25788277
    15. We show that some are new substrates of the anaphase-promoting complex/cyclosome and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading PMID: 24857844
    16. Our data underscore KIFC1 as a putative biomarker that predicts worse prognosis, poor overall survival and may serve as a potential marker of onset of metastatic dissemination in ovarian cancer patients. PMID: 25028599
    17. Study demonstrates microtubule tip-tracking of human kinesin-14 HSET and show that EB1 is sufficient to induce this dynamic microtubule end localization. PMID: 24039245
    18. Acentrosomal spindle assembly mechanisms are hyperactive in cancer cells and promote HSET. PMID: 22946058
    19. we suggest that KIFC1 plays an essential role for bipolar MTOC formation and maintaining chromosomal stability in the mitosis . PMID: 23318213
    20. A case-control analysis was carried out by comparing the genotype distribution of six KIFC1 single-nucleotide polymorphisms between 93 aspirin exacerbated respiratory disease cases and 96 aspirin-tolerant asthma controls in a Korean population PMID: 22201025
    21. The intense placental expression of KIFC1 in syncytiotrophoblast and KIF17 in vascular endothelium suggests that both proteins might be important in a cargo-transport system. KIFC1 and KIF17 expression are increased of both in preeclamptia and diabetes. PMID: 19679349
    22. An analysis was made of the weak interactions of ADP-Unc104 and ADP-kinesin with microtubules and their inhibition by MAP2. PMID: 17326138
    23. HSET has a role in controlling spindle length by cross-linking and sliding microtubules. PMID: 19116309
    24. High KIFC1 expression is associated with metastatic spread to the brain in primary non-small cell lung cancer. PMID: 19190132
    25. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Early endosome.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 6389

    OMIM: 603763

    KEGG: hsa:3833

    STRING: 9606.ENSP00000393963

    UniGene: Hs.436912



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