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KIF23 Recombinant Monoclonal Antibody

  • 中文名稱：
    KIF23 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA963424A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of kinesin family member 23 expression in HepG2 cells using kinesin family member 23 antibody. Green, isotype control; red, kinesin family member 23.
    • Immunocytochemical staining of HepG2 cells with Kinesin family member 23 antibody. Nuclei were stained blue with DAPI; Kinesin family member 23 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
    • Western blotting analysis using kinesin family member 23 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with kinesin family member 23 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    KIF23
  • 別名：
    KIF23; Kinesin Family Member 23; MKLP1; MKLP-1; KNSL5; Kinesin-Like 5 (Mitotic Kinesin-Like Protein 1); Kinesin-Like Protein KIF23; Congenital Dyserythropoietic Anemia, Type III; Mitotic Kinesin-Like Protein 1; Kinesin-Like Protein 5; CDAIII; CDAN3A; CDAN3; CDA3; CHO1
  • 反應種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human KIF23 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    22H10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
  • 基因功能參考文獻：
    1. Data show that p120-catenin interacts with kinesin family member 23 (MKLP1) to regulate focused rhoA GTP-binding protein (RhoA) activity during cytokinesis. PMID: 28004812
    2. KIF23 was found highly expressed in NSCLC PMID: 29277180
    3. Results show the mitotic regulators KIF23 and MIS18BP1 to be co-modified by SUMO and ubiquitin on inhibition of the proteasome and subsequently identified as novel RNF4 targets. PMID: 28951443
    4. Authors found that KIF23 was regulated by TCF-4 at transcriptionally level. Therefore, this evidence indicates KIF23 over-expression is associated with glioma malignancy and conferred a worse survival time in glioma. PMID: 27013586
    5. Data show high level of KIF23 expression in the majority of primary and metastatic lung cancer tissues or cell lines and associates with poor survival. PMID: 26775597
    6. In this study we show for the first time that KIF23 V1 and V2 have different localizations in hepatocellular carcinoma cells PMID: 26674738
    7. TRAF6 mediates ubiquitination of the midbody ring localized protein KIF23/MKLP1. PMID: 24128730
    8. Transcriptional regulation of KIF23 is mediated by TP53. PMID: 23650552
    9. KIF23 mutation is associated with congenital dyserythropoietic anemia type III. PMID: 23570799
    10. It was shown that, during cytokinesis, Arf6 is first accumulated around the cleavage furrow and, prior to abscission, recruited onto the Flemming body via interaction with MKLP1. PMID: 22522702
    11. The results of this study indicated that downregulation of KIF23 decreases proliferation of glioma cells and that KIF23 may be a novel therapeutic target in malignant glioma. PMID: 21904957
    12. Study shows that 14-3-3 protein binds centralspindlin when the kinesin-6 component MKLP1 is phosphorylated at S710; 14-3-3 prevents centralspindlin from clustering in vitro, and MKLP1 mutant that is unable to bind 14-3-3 forms aberrant clusters in vivo. PMID: 20451386
    13. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. The sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. PMID: 11877456
    14. Plk1 and CHO1/MKLP-1 interact to have a role in cytokinesis PMID: 15199097
    15. Recruitment of MKLP1 to the midzone/midbody by INCENP is a crucial step for the midbody formation and completion of cytokinesis in mammalian cells. PMID: 15796717
    16. Spatial restriction of Aurora B to the central spindle by MKlp2 regulates MKlp1 during cytokinesis in human cells. PMID: 16461284
    17. Cep55 directly binds to MKLP1 in vitro and associates with the MKLP1-MgcRacGAP centralspindlin complex in vivo PMID: 16790497
    18. Ectopic expression of a mutant Mklp-1 without the nuclear localization signal(s)(NLSs) leads to cell cycle arrest at cytokinesis, indicating that the NLSs are necessary for Mklp-1 to execute its normal function during cell division. PMID: 17198681
    19. study reports that mitotic complex genes Ect2, RacGAP, and MKLP1 are coordinately induced in S phase in proliferating T lymphocytes as well as in epithelial cells, depending upon activity of the CUX1 and E2F1 transcription factors PMID: 19015243
    20. MKLP1 interacts with CYK4, a GTPase activating protein for the Rho GTPase, to form the centralspindlin complex. Centralspindlin is a key regulator of Rho function and microtubule organisation during cytokinesis, and is essential for cell division. PMID: 11782313

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  • 亞細胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody, Midbody ring. Note=Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
  • 數據庫鏈接：

    HGNC: 6392

    OMIM: 605064

    KEGG: hsa:9493

    STRING: 9606.ENSP00000260363

    UniGene: Hs.270845