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  4. GTPBP4 Recombinant Monoclonal Antibody

GTPBP4 Recombinant Monoclonal Antibody

  • 中文名稱：
    GTPBP4 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA781541A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: MCF7 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), A549 whole cell lysate(30μg), A2780 whole cell lysate(30μg), COLO205 whole cell lysate(30μg)
      All lanes:GTPBP4 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 74 kDa
      Observed band size: 80 kDa
      Exposure time：1min
    • IHC image of CSB-RA781541A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA781541A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    GTPBP4
  • 別名：
    Chronic renal failure gene protein antibody; CRFG antibody; G protein binding protein CRFG antibody; GTP binding protein 4 antibody; GTP-binding protein NGB antibody; Gtpbp4 antibody; NGB antibody; NOG1 antibody; NOG1_HUMAN antibody; Nucleolar GTP binding protein 1 antibody; Nucleolar GTP-binding protein 1 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide from human GTPBP4 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    8F2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in the biogenesis of the 60S ribosomal subunit.
  • 基因功能參考文獻(xiàn)：
    1. GTPBP4 promotes colorectal cancer metastasis by disrupting actin cytoskeleton, which is mediated by the reduced RhoA activity.Up-regulation of GTPBP4 in colorectal carcinoma is responsible for tumor metastasis. PMID: 27720713
    2. NGB is a tumor suppressor that regulates and requires merlin to suppress cell proliferation PMID: 17210637
  • 亞細(xì)胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family, NOG subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 21535

    KEGG: hsa:23560

    STRING: 9606.ENSP00000354040

    UniGene: Hs.215766