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  4. GEMIN2 Recombinant Monoclonal Antibody

GEMIN2 Recombinant Monoclonal Antibody

  • 中文名稱：
    GEMIN2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA124739A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of GEMIN2 expression in HepG2 cells using GEMIN2 antibody. Green, isotype control; red, GEMIN2.
    • Immunocytochemical staining of HepG2 cells with GEMIN2 antibody. Nuclei were stained blue with DAPI; GEMIN2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using GEMIN2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GEMIN2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    GEMIN2
  • 別名：
    GEMIN2; Gem Nuclear Organelle Associated Protein 2; SIP1; Survival Of Motor Neuron Protein Interacting Protein 1; Gem-Associated Protein; Component Of Gems; Gemin-2; Survival Of Motor Neuron Protein-Interacting Protein 1; Gem (Nuclear Organelle) Associated Protein 2; SMN Interacting Protein 1-Delta; SMN-Interacting Protein 1; SIP1-Delta
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human GEMIN2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21A5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound.
  • 基因功能參考文獻(xiàn)：
    1. Specific mutations in the yeast Sm protein ring expose a requirement for assembly factor Brr1, a homolog of human Gemin2. PMID: 27974620
    2. The authors propose that Gemin2 is a versatile hub for ribonucleoprotein exchange that functions broadly in RNA metabolism. PMID: 26828962
    3. investigate the oligomeric nature of the SMN.Gemin2 complexes from humans and fission yeast (hSMN.Gemin2 and ySMN.Gemin2) PMID: 26092730
    4. Two monoclonal antibodies against SMN (survival-of-motor-neurons) protein bind to its site of interaction with gemin2. PMID: 23939045
    5. several conserved SMN residues, including the sites of two SMA patient mutations, are not required for binding to Gemin2. Instead, they form a conserved SMN/Gemin2 surface that may be functionally important for snRNP assembly. PMID: 22607171
    6. Overexpression of SIP1 and downregulation of E-cadherin is associated with delayed neck metastasis in stage I/II oral tongue squamous cell carcinoma after partial glossectomy PMID: 21913013
    7. purified SMN-GEMIN2 fusion protein enhanced the RAD51-mediated homologous pairing much more efficiently than GEMIN2 alone PMID: 21732698
    8. Study identified Gemin2 as the protein that binds a pentamer of Sm proteins comprised of SmD1/D2 and SmF/E/G; the crystal structure of this complex bound to SMN's Gemin2 binding domain to 2.5 A was determined. PMID: 21816274
    9. Novel monoclonal antibodies detect SIP1 in the cytoplasm of human cells from multiple tumor tissue arrays. PMID: 20515682
    10. This study demonstrated that miR-141 levels correlate inversely with SIP1 protein levels as well as cell migration and invasion of CRC cells; SIP1 was identified as a functional target of miR-141. PMID: 19830559
    11. This study supports the rationale for developing SIP1 as a potential therapeutic and diagnostic target for gliomas PMID: 19806322
    12. SIP1 appears to stabilize functional multimer forms of IN, thereby promoting the assembly of IN and RT on viral RNA to allow efficient reverse transcription, which is a prerequisite for efficient HIV-1 infection PMID: 19915660
    13. Spinal muscular atrophy and amyotropic lateral sclerosis patients have decreased SIP 1-alpha and increased SIP 1-beta expression levels compared to normal tissues. PMID: 11943600
    14. SIP1 determines the capacity for snRNP assembly: biochemical deficiency in spinal muscular atrophy. PMID: 15964810
    15. binds to HIV-1 integrase and facilitates viral cDNA synthesis and subsequent steps that precede integration in vivo PMID: 16731905
    16. Gemin2 plays an important role in small nuclear ribonucleoprotein assembly through the stabilization of the survival of motor neuron oligomer/complex via novel self-interaction PMID: 17308308
    17. relationship between the feature of gene mutations and single nucleotide polymorphisms of SIP1 gene and HSCR PMID: 18247312
    18. We found upregulation of mRNA for transcription factors Snail, Slug, Twist, and SIP1 in spindle cell carcinoma when compared to squamous cell carcinoma. PMID: 19381684

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  • 亞細(xì)胞定位：
    Nucleus, gem. Cytoplasm. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.
  • 蛋白家族：
    Gemin-2 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 10884

    OMIM: 602595

    KEGG: hsa:8487

    STRING: 9606.ENSP00000308533

    UniGene: Hs.652307



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