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DUT Recombinant Monoclonal Antibody

  • 中文名稱：
    DUT Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA985913A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of DUT expression in Hela cells using DUT antibody. Green, isotype control; red, DUT.
    • Immunocytochemical staining of Hela cells with DUT antibody. Nuclei were stained blue with DAPI; DUT was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
    • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using DUT antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Immunohistochemistry was performed on paraffin-embedded mouse brain using DUT antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Immunohistochemistry was performed on paraffin-embedded mouse heart using DUT antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Western blotting analysis using DUT antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DUT antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    DUT
  • 別名：
    DUT; Deoxyuridine Triphosphatase; DUTP Pyrophosphatase; DUTPase; Deoxyuridine 5'-Triphosphate Nucleotidohydrolase, Mitochondrial; DUTP Diphosphatase; EC 3.6.1.23; DUTP Nucleotidohydrolase; DUTPASE; BMFDMS
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human DUT protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    2G3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC, ICC, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
    IHC 1:100-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • 基因功能參考文獻(xiàn)：
    1. A single homozygous missense mutation (chr15.hg19:g.48,626,619A>G) located in the dUTPase (DUT) gene was associated with a syndrome of bone marrow failure and diabetes. PMID: 28073829
    2. Comparison of the cellular distribution of wild-type dUTPase with those of hyperphosphorylation- and hypophosphorylation-mimicking mutants suggests that phosphorylation at Ser11 leads to the exclusion of dUTPase from the nucleus. PMID: 24311590
    3. EBV-encoded dUTPase may act as an intercellular signaling molecule capable of modulating the cellular microenvironment PMID: 23894549
    4. Data sugget that inhibition of dUTPase is a mechanism-based therapeutic approach to significantly enhance the efficacy of thymidylate synthase (TS)-targeted chemotherapeutic agents. PMID: 22172489
    5. Repression of dUTPase induced specific expression level increments for thymidylate kinase and thymidine kinase, and also an increased sensitization to 5-fluoro-2'-deoxyuridine and 5-fluoro-uracil. PMID: 21780905
    6. High dUTP pyrophosphatase is associated with recurrence in colorectal cancer patients undergoing 5-fluorouracil adjuvant chemotherapy PMID: 20560137
    7. nuclear dUTPase may be a good biomarker for predicting prognosis in HCC patients after surgical resection PMID: 19968781
    8. significant differences between the human and Plasmodium dUTPases PMID: 19879316
    9. Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil. PMID: 12096336
    10. Nuclear dUTPase may contain a complex nuclear localization signal that is located throughout the entire protein. PMID: 12799180
    11. dUTPase staining provides a useful measure of cell proliferation distinct from that offered by Ki-67 labeling. It proved particularly useful for the evaluation of diffuse astrocytomas. PMID: 16325515
    12. kinetic model of the human dUTPase catalytic cycle PMID: 17848562
    13. determined the crystal structure of the enzyme:alpha,beta-imino-dUTP PMID: 17880943
    14. Establishment of a direct role for both mutant and wild-type forms of p53 in modulating dUTPase promoter activity. PMID: 19015155
    15. hinge proline destabilize human and Escherichia coli dUTPases without preventing trimeric organization PMID: 19302784

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  • 亞細(xì)胞定位：
    [Isoform 2]: Nucleus.; [Isoform 3]: Mitochondrion.
  • 蛋白家族：
    DUTPase family
  • 組織特異性：
    Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein leve
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3078

    OMIM: 601266

    KEGG: hsa:1854

    STRING: 9606.ENSP00000370376

    UniGene: Hs.527980