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  4. DFFA Recombinant Monoclonal Antibody

DFFA Recombinant Monoclonal Antibody

  • 中文名稱：
    DFFA Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA112519A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of DFFA expression in HeLa cells using DFFA antibody. Green, isotype control; red, DFFA.
    • Western blotting analysis using DFFA antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DFFA antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    DFFA; DNA Fragmentation Factor Subunit Alpha; DFF-45; DFF45; ICAD; DFF1; DNA Fragmentation Factor, 45kDa, Alpha Polypeptide; DNA Fragmentation Factor 45KDa Subunit; Inhibitor Of CAD; DNA Fragmentation Factor, 45 KD, Alpha Polypeptide; DNA Fragmentation Factor, 45 KD, Alpha Subunit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human DFFA protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    12F1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Inhibitor of the caspase-activated DNase (DFF40).
  • 基因功能參考文獻：
    1. Glandular, menopause-independent DFF40, DFF45, and Bcl-2 overexpression may play an important role in the pathogenesis of endometrial polyps and benign endometrial hyperplasia PMID: 28914671
    2. evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients PMID: 29091952
    3. Data show that the caspase-activated DNase (CAD) is activated when caspases cleave its endogenous inhibitor ICAD, resulting in the characteristic DNA laddering of apoptosis. PMID: 26106156
    4. Data suggest that DFF45 gene silencing, when applied in combination with doxorubicin, may offer a therapeutic strategy for the treatment of breast cancer. PMID: 24277473
    5. ICAD deficiency was associated with severe genomic instability. PMID: 23451280
    6. mRNA splicing is actively driven toward the pro-apoptotic isoforms of Bim, Bcl-x, and ICAD in Pnn-depleted MCF-7 cells. PMID: 22454513
    7. The DFF45 level in human endometrium corresponds to the respective phase of the menstrual cycle and decreases significantly after menopause. PMID: 22378161
    8. study reveals a previously unrecognized function of miR-145 in DFF45 processing, which may underlie crucial aspects of cancer biology PMID: 20687965
    9. The heterodimer, DFF40-DFF45, is localized to the chromatin fraction under apoptotic as well as non-apoptotic conditions. PMID: 19882353
    10. DFF45 at chromosome 1 reveal rare allelic variants in neuroblastoma tumors PMID: 11870543
    11. NMR solution structure of the C-terminal domain of DFF45, which is essential for its chaperone-like activity PMID: 12144788
    12. Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes. PMID: 12181128
    13. subunit structures and stoichiometries in human cells before and after induction of apoptosis PMID: 12748178
    14. Hepatitis C virus core protein increases a steady-state level of ICAD protein, possibly through enhancing its promoter activity PMID: 14675622
    15. apoptotic DNA fragmentation factor is required for the maintenance of genetic stability and may play a role in tumor suppression PMID: 16432220
    16. plays an important and P53-independent role in maintaining chromosome stability and suppressing tumor development PMID: 16619042
    17. demonstrate the cellular mechanisms of neuronal cell degeneration induced via c-Jun-N-terminal kinases and caspase-dependent signaling PMID: 17645689
    18. C-terminal region of each subunit, DFF40 (RLKRK) and DFF45 (KRAR), is essential for nuclear accumulation of the DFF complex. PMID: 17938174
    19. Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of caspase-2 in nuclear DNA fragmentation. PMID: 17945178
    20. The highest level of DFF45 endometrial expression was found during the early secretory cycle phase, and significantly lower DFF45 expression was found in the endometrium during the mid-secretory as compared to the early secretory cycle phase. PMID: 18292826
    21. identified a TATA-less region upstream of the transcription start site as a basal promoter of the ICAD gene. An E-Box motif, which binds transcription factors of the basic helix-loop-helix/leucine zipper family, is responsible for transcriptional activity PMID: 18500556
    22. MPP(+) did not change the total levels of c-Jun but enhanced phosphorylation of c-Jun at Ser73 and cleavage of DNA fragmentation factor 45, which were diminished by selegiline. PMID: 18805449
    23. A decreased level of DFF45 observed in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression. PMID: 19535198

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  • 亞細胞定位：
    Cytoplasm.
  • 數據庫鏈接：

    HGNC: 2772

    OMIM: 601882

    KEGG: hsa:1676

    STRING: 9606.ENSP00000366237

    UniGene: Hs.484782