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CLTC/CLTCL1 Recombinant Monoclonal Antibody

  • 中文名稱：
    CLTC/CLTCL1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA172477A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(30μg), K562 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), HL-60 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HEK293 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Rat brain tissue lysate(30μg)
      All lanes: Clathrin heavy chain 1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 192, 187 kDa
      Observed band size: 192 kDa
      Exposure time：10s
    • IHC image of CSB-RA172477A0HU diluted at 1:100 and staining in paraffin-embedded mouse kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA172477A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing MCF-7 cells stained with CSB-RA172477A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    CLTC/CLTCL1
  • 別名：
    CHC17 antibody; Clathrin heavy chain antibody; Clathrin heavy chain 1 antibody; Clathrin heavy chain 2 antibody; Clathrin heavy chain like 1 antibody; Clathrin heavy chain on chromosome 17 antibody; Clathrin heavy polypeptide antibody; CLH 17 antibody; CLH 22 antibody; CLH-17 antibody; CLH1_HUMAN antibody; CLH17 antibody; CLH22 antibody; CLTC antibody; CLTCL antibody; CLTCL1 antibody; CLTCL2 antibody; CLTD antibody; Hc antibody; KIAA0034 antibody; CLTCL1 antibody; CLH22 antibody; CLTCL antibody; CLTDClathrin heavy chain 2 antibody; Clathrin heavy chain on chromosome 22 antibody; CLH-22 antibody
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide from human CLTC/CLTCL1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    15H3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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