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  4. CLP1 Recombinant Monoclonal Antibody

CLP1 Recombinant Monoclonal Antibody

  • 中文名稱：
    CLP1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA940020A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of CLP1 expression in HeLa cells using CLP1 antibody. Green, isotype control; red, CLP1.
    • Immunocytochemical staining of Hela cells with CLP1 antibody. Nuclei were stained blue with DAPI; CLP1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using CLP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    CLP1
  • 別名：
    CLP1; Cleavage Factor Polyribonucleotide Kinase Subunit 1; HEAB; Cleavage And Polyadenylation Factor I Subunit 1; Polyribonucleotide 5'-Hydroxyl-Kinase Clp1; Pre-MRNA Cleavage Complex II Protein Clp1; Polyadenylation Factor Clp1; Polynucleotide Kinase Clp1; EC 2.7.1.78; HClp1; CLP1, Cleavage And Polyadenylation Factor I Subunit, Homolog (S. Cerevisiae); Polyribonucleotide 5'-Hydroxyl-Kinase; Homolog Of Yeast CFIA Subunit Clp1p; ATP/GTP-Binding Protein; ATP/GTPbinding Protein; HCLP1
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human CLP1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2B11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
  • 基因功能參考文獻：
    1. Study reports the identification of a human CLP1 mutation that impairs tRNA splicing in vitro and causes a neurological syndrome involving both the central nervous system (CNS) and peripheral nervous system (PNS). PMID: 24766809
    2. Data link tRNA maturation to neuronal development and neurodegeneration through defective CLP1 function in humans. PMID: 24766810
    3. the kinase hClp1 phosphorylates and licenses synthetic siRNAs to become assembled into RISC for subsequent target RNA cleavage PMID: 17495927
    4. Expression of hClp1 in budding yeast can complement conditional and lethal mutations in the essential 5'-OH RNA kinase module of yeast or plant tRNA ligases. PMID: 18648070
    5. HEAB is a pre-mRNA 3'-end processing factor, which bridges two other pre-mRNA processing factors (CF Im and CPSF) and is involved in cleavage of the pre-mRNA. It can be UV-crosslinked to GTP. PMID: 11060040

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  • 相關疾?。?/div>
    Pontocerebellar hypoplasia 10 (PCH10)
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Clp1 family, Clp1 subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 16999

    OMIM: 608757

    KEGG: hsa:10978

    STRING: 9606.ENSP00000434995

    UniGene: Hs.523687