CHMP3 Recombinant Monoclonal Antibody
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中文名稱:CHMP3 Recombinant Monoclonal Antibody
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貨號(hào):CSB-RA591955A0HU
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規(guī)格:¥1320
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圖片:
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IHC image of CSB-RA591955A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA591955A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Overlay Peak curve showing MCF-7 cells stained with CSB-RA591955A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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其他:
產(chǎn)品詳情
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Uniprot No.:
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基因名:CHMP3
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別名:VPS 24 antibody; VPS24 antibody; CGI 149 antibody; CGI149 protein antibody; Charged multivesicular body protein 3 antibody; CHMP3 antibody; CHMP3_HUMAN antibody; Chromatin modifying protein 3 antibody; Chromatin-modifying protein 3 antibody; HGNC:29865 antibody; hVps24 antibody; NEDF antibody; Neuroendocrine differentiation factor antibody; Vacuolar protein sorting 24 (yeast) antibody; Vacuolar protein sorting 24 homolog (S. cerevisiae) antibody; Vacuolar protein sorting associated protein 24 antibody; Vacuolar protein sorting-associated protein 24 antibody; vps24 antibody
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反應(yīng)種屬:Human
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免疫原:A synthesized peptide from human CHMP3 protein
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
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克隆類(lèi)型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號(hào):24F6
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, IHC, FC
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推薦稀釋比:
Application Recommended Dilution IHC 1:50-1:200 FC 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress-mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.
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亞細(xì)胞定位:Cytoplasm, cytosol. Membrane; Lipid-anchor. Endosome. Late endosome membrane. Note=Localizes to the midbody of dividing cells.
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蛋白家族:SNF7 family
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組織特異性:Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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數(shù)據(jù)庫(kù)鏈接:
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