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  4. ARF5 Recombinant Monoclonal Antibody

ARF5 Recombinant Monoclonal Antibody

  • 中文名稱：
    ARF5 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA198186A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of ADP ribosylation factor 5 expression in HepG2 cells using ADP ribosylation factor 5 antibody. Green, isotype control; red, ADP ribosylation factor 5.
    • Immunocytochemical staining of HepG2 cells with ADP ribosylation factor 5 antibody. Nuclei were stained blue with DAPI; ADP ribosylation factor 5 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using ADP ribosylation factor 5 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADP ribosylation factor 5 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    ARF5; ADP Ribosylation Factor 5; ADP-Ribosylation Factor 5
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human ARF5 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2H5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.; (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
  • 基因功能參考文獻：
    1. GORAB missense mutation disrupt ARF5 binding in Golgi apparatus in patients, with genetic skin diseases. PMID: 26000619
    2. BRAG2 acts at clathrin-coated pits to promote integrin internalization by activating Arf5 and suggest a previously unrecognized role for Arf5 in clathrin-mediated endocytosis of specific cargoes. PMID: 22815487
    3. The lipid droplets deposition and the cellular triacylglycerol content are significantly increased by siRNA-mediated depletion of Arf5. PMID: 22185782
  • 亞細胞定位：
    Golgi apparatus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor.
  • 蛋白家族：
    Small GTPase superfamily, Arf family
  • 數據庫鏈接：

    HGNC: 658

    OMIM: 103188

    KEGG: hsa:381

    STRING: 9606.ENSP00000000233

    UniGene: Hs.653202



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