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  4. ALKBH2 Recombinant Monoclonal Antibody

ALKBH2 Recombinant Monoclonal Antibody

  • 中文名稱：
    ALKBH2 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA949939A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of ALKBH2 expression in HepG2 cells using ALKBH2 antibody. Green, isotype control; red, ALKBH2.
    • Immunocytochemical staining of HepG2 cells with ALKBH2 antibody. Nuclei were stained blue with DAPI; ALKBH2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using ALKBH2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALKBH2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    ALKBH2
  • 別名：
    ALKBH2; AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase; ABH2; Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 2; Alkylated DNA Repair Protein AlkB Homolog 2; DNA Oxidative Demethylase ALKBH2; MGC90512; Oxy DC1; AlkB, Alkylation Repair Homolog 2 (E. Coli); AlkB, Alkylation Repair Homolog 2; 2OG-Fe(II) Oxy DC1; EC 1.14.11.33
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human ALKBH2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5H12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
  • 基因功能參考文獻：
    1. These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
    2. studies reveal the ALKBH2 binding interface of PCNA and indicate that both germline and somatic ALKBH2 variants could have cellular effects on ALKBH2 function in DNA repair. PMID: 26408825
    3. It was shown for first time that DNA glycosylase ALKBH2 can repair DNA adduct 1,N2-ethenoguanine. PMID: 25797601
    4. ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks in the rDNA genes. PMID: 23972994
    5. Overexpression of ALKBH2 is associated with enhanced resistance to temozolomide in glioblastoma. PMID: 23258843
    6. ALKBH2 is an upstream molecule of the oncoprotein, MUC1, and regulates cell cycle and EMT, resulting in progression of urothelial carcinomas. PMID: 23279696
    7. combination of duplex interrogation and oxidation chemistry allows ALKBH2 to detect and process diverse lesions efficiently and correctly PMID: 22659876
    8. The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
    9. X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2 PMID: 21510633
    10. ABH2 is downregulated in a subset of gastric cancers, and might be involved in the molecular mechanism of gastric cancer through inhibiting the proliferation of gastric cancer cells. PMID: 21155885
    11. This work has provided a detailed understanding of the structural features of the single-stranded DNA and double-stranded DNA preferences of ABH2 and ABH3. PMID: 20714506
    12. Divergent sequences outside of the active site determine substrate specificities of ABH2. PMID: 20525795
    13. Tumour protein (TP53) is directly involved by binding to the promoter of ALKBH2 in mediating photofrin-mediaated photodynamic therapy in U87 glioma cells. PMID: 20661249
    14. first crystal structure of ABH2 PMID: 18432238
    15. ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions PMID: 18519673
    16. Mutations of ABH2 is associated with pediatric brain tumors. PMID: 19290481

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  • 亞細胞定位：
    Nucleus. Note=Detected in replication foci during S-phase.
  • 蛋白家族：
    AlkB family
  • 組織特異性：
    Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
  • 數據庫鏈接：

    HGNC: 32487

    OMIM: 610602

    KEGG: hsa:121642

    STRING: 9606.ENSP00000343021

    UniGene: Hs.374458