ALAS1 Recombinant Monoclonal Antibody
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中文名稱:ALAS1 Recombinant Monoclonal Antibody
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貨號:CSB-RA220019A0HU
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規格:¥1320
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圖片:
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Flow cytometric analysis of ALAS1 expression in HAP-1 cells using ALAS1 antibody. Green, isotype control; red, ALAS1.
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Immunocytochemical staining of HAP-1 cells with ALAS1 antibody. Nuclei were stained blue with DAPI; ALAS1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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Western blotting analysis using ALAS1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALAS1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他:
產品詳情
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Uniprot No.:
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基因名:ALAS1
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別名:ALAS1; 5'-Aminolevulinate Synthase 1; ALAS-H; ALAS3; 5-Aminolevulinate Synthase, Non-Specific, Mitochondrial; Aminolevulinate, Delta-, Synthase 1; 5-Aminolevulinic Acid Synthase 1; Delta-Aminolevulinate Synthase 1; Delta-ALA Synthase 1; EC 2.3.1.37; ALASH; ALAS; 5-Aminolevulinate Synthase, Nonspecific, Mitochondrial; Migration-Inducing Protein 4; EC 2.3.1; MIG4
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反應種屬:Human, Mouse, Rat
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免疫原:Recombinant Human ALAS1 protein
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免疫原種屬:Homo sapiens (Human)
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標記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號:14D11
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產品提供形式:Liquid
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應用范圍:ELISA, WB, FC, ICC
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推薦稀釋比:
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關產品
靶點詳情
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基因功能參考文獻:
- The mutation in CLPX inactivates its ATPase activity, resulting in coassembly of mutant and WT protomers to form an enzyme with reduced activity. The presence of low-activity CLPX increases the posttranslational stability of ALAS, causing increased ALAS protein and ALA levels, leading to abnormal accumulation of PPIX. PMID: 28874591
- the heme-binding site in the N-terminal region of the mature ALAS1 protein is also necessary for the heme-dependent oxidation of ALAS1. PMID: 27496948
- ALAS1 mRNA and activity were elevated approximately ~3- and 5-fold, and HMB synthase activity was approximately half-normal (~42%) PMID: 26062020
- The -853T variant functions as an enhancer in the presence of estrogen and speculates that the -1253A variant reduces transcription activity. PMID: 19656447
- These results indicate that ALAS1 is a novel NR5A-target gene and participates in steroid hormone production. PMID: 23024262
- Lon peptidase 1 (LONP1)-dependent breakdown of mitochondrial 5-aminolevulinic acid synthase protein by heme in human liver cells. PMID: 21659532
- REVIEW:active site and mechanistic analysis, protein folding, structure and function. PMID: 11929042
- REVIEW: mechanisms involving ALAS deficiency, point mutations, post translational processing, and complex formation with succinyl CoA synthetase subunit B in the pathogenesis of hereditary sideroblastic anemia. PMID: 11929048
- ALAS expression is regulated by AP-1 complex through sequestration of cAMP-response element protein (CRE)-binding protein (CBP) coactivator in human cells PMID: 12433930
- ALAS gene expression is regulated by Hepatic nuclear factor 3 and nuclear factor 1 PMID: 15123725
- First described frameshift ALAS2 mutation, CD506-507 (-C). PMID: 15477213
- in the liver of Acute liver failure patients, there may be an increase in free heme concentration which down-regulating ALAS1 gene expression PMID: 15547665
- Alternative splicing of human ALAS1 generates two mRNAs with different 5'-UTRs: a major one, where exon 1B is omitted, and a minor form containing exon 1B. PMID: 15710391
- 5-aminolevulinate synthase gene repression by the potent tumor promoter, TPA, involves multiple signal transduction pathways PMID: 15797241
- Expression of candidate genes HPRT1 and ALAS1 in malignant and non-malignant prostate tissue samples after microdissection. PMID: 17628775
- Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin. PMID: 18719978
- In this study, we show significant reductions of the rate-limiting enzymes involved in heme biosynthesis, ALAS1 in the postmortem cortex of Alzheimer's disease subjects, providing additional evidence of abnormal heme homeostasis in Alzheimer's disease. PMID: 19477221
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亞細胞定位:Mitochondrion matrix.
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蛋白家族:Class-II pyridoxal-phosphate-dependent aminotransferase family
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