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  4. ACAT1 Recombinant Monoclonal Antibody

ACAT1 Recombinant Monoclonal Antibody

  • 中文名稱：
    ACAT1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA089464A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of acetyl-CoA acetyltransferase 1 expression in HT-1080 cells using acetyl-CoA acetyltransferase 1 antibody. Green, isotype control; red, acetyl-CoA acetyltransferase 1.
    • Immunocytochemical staining of HT-1080 cells with Acetyl-CoA acetyltransferase 1 antibody. Nuclei were stained blue with DAPI; Acetyl-CoA acetyltransferase 1 with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using acetyl-CoA acetyltransferase 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acetyl-CoA acetyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    ACAT1
  • 別名：
    ACAT1; Acetyl-CoA Acetyltransferase 1; THIL; ACAT; Acetyl-CoA Acetyltransferase, Mitochondrial; Acetyl-Coenzyme A Acetyltransferase 1; Acetoacetyl Coenzyme A Thiolase; Acetoacetyl-CoA Thiolase; EC 2.3.1.9; MAT; T2; Mitochondrial Acetoacetyl-CoA Thiolase; Testicular Tissue Protein Li 198; EC 2.3.1
  • 反應種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human ACAT1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    11G6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
  • 基因功能參考文獻：
    1. High ACAT1 expression is associated with breast cancer. PMID: 30092298
    2. Insulin promotes progression of colon cancer by upregulation of ACAT1. PMID: 29793481
    3. ACAT1 exonic mutations that affect ESE sequences may result in aberrant splicing. This may affect the activity of mitochondrial acetoacetyl-CoA thiolase. PMID: 27748876
    4. compound heterozygous of ACAT1 gene mutations probably underlie the beta-ketothiolase deficiency in our patient PMID: 27264805
    5. Data indicate that acetyl-CoA acetyltransferase (ACAT1) and malate dehydrogenase (MDH2) are involved in various drug-resistance-forming mechanisms. PMID: 25639359
    6. the pyruvate dehydrogenase complex is regulated by Tyr phosphorylation of PDP1, which toggles recruitment between ACAT1 and SIRT3 PMID: 24486017
    7. these findings indicate that ACAT1 expression could serve as a potential prognostic marker in prostate cancer, specifically in differentiating indolent and aggressive forms of cancer. PMID: 24311408
    8. ACAT1 expression is substantially elevated in human castration-resistant metastatic prostate cancer tissues. PMID: 23443136
    9. Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085
    10. We herein identified a common mutation, R208X, in Vietnamese patients. We identified R208X homozygously in six patients and heterozygously in two patients among eight Vietnamese patients. PMID: 20156697
    11. the siblings with the same T2 gene mutations present different clinical severity of T2 deficiency PMID: 20046049
    12. Single base substitutions at the initiator codon produced wild-type T2 polypeptide to various degrees PMID: 12754704
    13. mild mutations (A132G, D339-V340insD) retained some residual T2 activity PMID: 15128923
    14. Cloning and sequencing long range PCR products revealed a 6.4kb deletion. Alu element-mediated unequal homologous recombination between an Alu-Sx in intron 1 and another Alu-Y in intron 4 appears to be responsible for this deletion. PMID: 16935016
    15. E252del is a temperature-sensitive K(m) mutant. PMID: 17236799
    16. Crystallographic and kinetic studies were made on mitochondrial acetyl-CoA thiolase: the importance of potassium and chloride ions were noted for its structure and function. PMID: 17371050
    17. A point mutation was responsible for the aberrant RNA splicing of the mitochondrial acetoacetyl-CoA thiolase gene. PMID: 18511318
    18. sequence recognition by the catalytic domain differs between hT2 and hT10 in that hT10 requires a pre-existing GalNAc residue while hT2 does not PMID: 18562306
    19. I/D polymorphism of ACE gene and AC AT1 gene influence the development of hypertension and Left Ventricular Hypertrophy in Hemodialysis patients. PMID: 19112833

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  • 相關疾病：
    3-ketothiolase deficiency (3KTD)
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    Thiolase family
  • 數據庫鏈接：

    HGNC: 93

    OMIM: 203750

    KEGG: hsa:38

    STRING: 9606.ENSP00000265838

    UniGene: Hs.232375