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recQ Antibody

Rare Species
  • 中文名稱：
    recQ兔多克隆抗體
  • 貨號：
    CSB-PA17979A0Rb
  • 規格：
    ￥440
  • 圖片：
    • Western blot
      All lanes: ATP-dependent DNA helicase RecQ antibody at 2μg/ml + DH5a whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 68 kDa
      Observed band size: 68 kDa
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Escherichia coli recQ Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    recQ
  • 別名：
    recQ antibody; b3822 antibody; JW5855 antibody; ATP-dependent DNA helicase RecQ antibody; EC 3.6.4.12 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli ATP-dependent DNA helicase RecQ protein (2-606AA)
  • 免疫原種屬：
    Escherichia coli
  • 標記方式：
    Non-conjugated

    本頁面中的產品，recQ Antibody (CSB-PA17979A0Rb)，的標記方式是Non-conjugated。對于recQ Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA17979B0Rb recQ Antibody, HRP conjugated ELISA
    FITC CSB-PA17979C0Rb recQ Antibody, FITC conjugated
    Biotin CSB-PA17979D0Rb recQ Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.
  • 基因功能參考文獻：
    1. To illustrate the single-molecule unwinding assay and the analysis routine, we provide DNA unwinding measurements of Escherichia coli RecQ helicase under a variety of conditions (Na+, ATP, temperature, and DNA substrate geometry). PMID: 27131595
    2. Data show that the single-stranded DNA binding protein (SSB)-RecQ helicase (RecQ) interaction changes the binding mode of SSB, thereby allowing RecQ to gain access to ssDNA and facilitating DNA unwinding. PMID: 29059328
    3. the mechanochemical coupling of E. coli RecQ appears to be independent of HRDC-ssDNA and HRDC-RecA core interactions, which may play roles in more specialized functions of the enzyme. PMID: 26067769
    4. Thus, bacterial type 1A topos maintain the stability of the genome (i) by preventing over-replication originating from oriC (topo I alone) and R-loops and (ii) by acting with RecQ. PMID: 25102178
    5. The study found that the interaction of RecQ with DNA results in a drastic acceleration of the rate-limiting ATP cleavage step, which occurs productively due to subsequent rapid phosphate release. PMID: 25539922
    6. The results shed light on the nature of conformational changes leading to processive ssDNA translocation and multistranded DNA processing by RecQ helicases. PMID: 24403069
    7. Results describe the mechanism of DNA unwinding by the Escherichia coli RecQ helicase using a new sensitive helicase assay based on fluorescence cross-correlation spectroscopy (FCCS) with two-photon excitation. PMID: 20048388
    8. The aromatic-rich loop in RecQ is critical for coupling ATPase and DNA-binding/unwinding activities. PMID: 16340008
    9. E.coli RecQ functions as a monomer and some of the Superfamily 2 helicases may function through an "inchworm" mechanism PMID: 16507576
    10. RecQ promotes the net accumulation of bimolecular recombination intermediates in vivo, indicating a second paradigm for the in vivo effect of RecQ-like proteins. PMID: 17466628
    11. SSB is a broadly conserved RecQ-binding protein PMID: 17483090
    12. These results support a model in which RecQ has evolved an SSB-C-terminal binding site on its winged-helix domain as an adaptation that aids its cellular functions on SSB/DNA nucleoprotein substrates. PMID: 19150358

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  • 蛋白家族：
    Helicase family, RecQ subfamily
  • 數據庫鏈接：


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