TCEA3 Antibody

中文名稱：

TCEA3兔多克隆抗體
貨號：

CSB-PA023271LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA023271LA01HU diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA023271LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) TCEA3 Polyclonal antibody
Uniprot No.：

O75764
基因名：

TCEA3
別名：

Rhabdomyosarcoma antigen MU RMS 40.22 antibody; Tcea3 antibody; TCEA3_HUMAN antibody; TFIIS antibody; TFIIS.H antibody; Transcription elongation factor A (SII) 3 antibody; Transcription elongation factor A protein 3 antibody; Transcription elongation factor A3 antibody; Transcription elongation factor S-II protein 3 antibody; Transcription elongation factor TFIIS.h antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Transcription elongation factor A protein 3 protein (79-169AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，TCEA3 Antibody (CSB-PA023271LA01HU)，的標記方式是Non-conjugated。對于TCEA3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA023271LB01HU	TCEA3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA023271LC01HU	TCEA3 Antibody, FITC conjugated
Biotin	CSB-PA023271LD01HU	TCEA3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
基因功能參考文獻：
1. Findings reveal a novel mechanism by which p53 utilizes TFIIS.h to selectively promote the transcriptional elongation of the bax gene, upsurging cell death in response to severe DNA damage. PMID: 27005522
2. TCEA3 attenuates the proliferation and induces apoptosis of gastric cancer cells PMID: 26498664
3. Tcea3 binds to TGF-beta I receptor and activates TGF-beta signaling pathway. PMID: 23357533
4. Study demonstrates a strong synergy between hPAF1C and elongation factor SII/TFIIS and an underlying mechanism involving direct hPAF1C-SII interactions and cooperative binding to RNA polymerase II. PMID: 20178742
亞細胞定位：

Nucleus.
蛋白家族：

TFS-II family
數據庫鏈接：

HGNC: 11615

OMIM: 604128

KEGG: hsa:6920

STRING: 9606.ENSP00000406293

UniGene: Hs.446354