Western Blot
Positive WB detected in: recombinant protein
All lanes:NDI1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 33.5 kDa
Observed band size: 34 kDa
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期:
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情
功能:
Catalyzes the oxidation of NADH generated inside the Mitochondrion.
基因功能參考文獻(xiàn):
Ubiquinone binding site of yeast NADH dehydrogenase Ndi1 has been described. PMID: 29402945
Ndi1 associations took place with complexes III and IV suggest the formation of respirasome-like structures. PMID: 26173916
Ndi1 assimilates electrons to the electron transport chain and initiates the respiration process to make ATP, whereas under stresses, it cleaves the toxicity-sequestering N-terminal cap, is released from the mitochondria, and becomes a cell killer. PMID: 22993213
The structure of the yeast NADH dehydrogenase (Ndi1) reveals overlapping binding sites for water- and lipid-soluble substrates PMID: 22949654
Our data indicate a central role for Complex I dysfunction in pink1-associated defects, and our genetic analyses with heterologous ETC enzymes PMID: 22242018
Perfusion of TAT-Ndi1 before 30 min no-flow ischemia and up to 2 hr reperfusion suppressed ROS production and preserved ATP stores. Importantly, TAT-Ndi1 infused before ischemia reduced infarct size by 62%. PMID: 21339825
the Ndi1 reaction proceeds through a ternary complex (not a ping-pong) mechanism. The bound UQ keeps oxygen away from the reduced flavin. PMID: 21220430
Yeast NDI1 expression in Drosophila mitigated the aging associated decline in respiratory capacity and the accompanying increase in mitochondrial reactive oxygen species production, and resulted in decreased accumulation of markers of oxidative damage. PMID: 20435911
report that expression of Ndi1 in fly mitochondria leads to an increase in NADH-ubiquinone oxidoreductase activity, oxygen consumption, and ATP levels. PMID: 20089120
identify the ubiquinone binding site in Ndi1 PMID: 20192260
Disruption of NDI1 decreases ROS production and elongates the chronological life span of yeast, accompanied by the loss of survival fitness. PMID: 16436509
Oxidative stress was significantly decreased when the cells were transduced with NDI1. Furthermore, mitochondria from the NDI1-transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors PMID: 17055488
data suggest that Ndi1 bears at least two distinct Q sites: one for bound Q and the other for catalytic Q. PMID: 17200125
NDI1 enzyme can improve the oxidative phosphorylation capacity in cells carrying the G11778A mutation and protect the cells from oxidative stress and cell death. PMID: 17320357
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亞細(xì)胞定位:
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note=Bound to the mitochondrial inner membrane facing the matrix site.