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WTAP Monoclonal Antibody

  • 中文名稱：
    WTAP Monoclonal Antibody
  • 貨號：
    CSB-MA918925
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of WTAP expression in HeLa cells using WTAP antibody. Green, isotype control; red, WTAP.
    • Immunocytochemical staining of Hela cells with WTAP associated protein antibody. Nuclei were stained blue with DAPI; WTAP associated protein was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. Permeabilization: Triton.
    • Western blotting analysis using WTAP antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WTAP antibody and HRP-conjugated goat mouse secondary antibody respectively. Image was developed using FeQ? ECL Substrate Kit .
  • 其他：

產品詳情

  • 產品名稱：
    Mouse anti-Homo sapiens (Human) WTAP Monoclonal antibody
  • Uniprot No.：
  • 基因名：
    WTAP
  • 別名：
    WTAP; WT1 Associated Protein; KIAA0105; Mum2; Wilms Tumor 1-Associating Protein; Wilms Tumor 1 Associated Protein; Pre-MRNA-Splicing Regulator WTAP; Female-Lethal(2)D Homolog; MGC3925; HFL(2)D; Putative Pre-MRNA Splicing Regulator Female-Lethal(2D); Wilms' Tumour 1-Associating Protein; WT1-Associated Protein; PNAS-132
  • 宿主：
    Mouse
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human WTAP protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    9F7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2500
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
  • 基因功能參考文獻：
    1. WTAP was over-expressed in glioma tissues, and the expression was closely correlated with glioma grade. PMID: 27370540
    2. WTAP as a regulatory subunit is required for formation of a functional m6A methyltransferase complex including METTL3 and METTL14, which play an important role in the regulation of gene expression and alternative splicing. PMID: 24407421
    3. WTAP regulates migration and invasion of cholangiocarcinoma cells. PMID: 23354623
    4. WTAP has an important role in abnormal proliferation and arrested differentiation of leukemia cells, and WTAP is a novel client protein of Hsp90 PMID: 24413322
    5. the WTAP complex is a novel component of the RNA processing machinery, implying an important role in both posttranscriptional control and cell cycle regulation. PMID: 24100041
    6. WTAP overexpression made cancer cells more tumorigenic PMID: 22957919
    7. WTAP level in poodocytes is a valuable alternative technique in the study of podocyte injury in diabetic nephropathy. PMID: 19969384
    8. WTAP protein involved in the morphogenesis of the genitourinaty tract and in the etiology of isolated hypospadias. PMID: 14675924
    9. WTAP and ATF3 Transcription Factor are activated in Wilms' tumor and accelerate tumorigenesis. PMID: 16912181
    10. WTAP as an essential factor for the stabilization of cyclin A2 mRNA, thereby regulating G2/M cell-cycle transition PMID: 17088532
    11. WTAP is a novel regulator of the cell cycle and cell survival and implicate a WTAP-WT1 axis as a novel pathway for controlling vascular smooth muscle cell phenotype. PMID: 17095724
    12. Nuclear degradation of Wilms tumor 1-associating protein and survivin splice variant switching underlie IGF-1-mediated survival PMID: 19605357

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  • 亞細胞定位：
    Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm.
  • 蛋白家族：
    Fl(2)d family
  • 組織特異性：
    Ubiquitously expressed.
  • 數據庫鏈接：

    HGNC: 16846

    OMIM: 605442

    KEGG: hsa:9589

    STRING: 9606.ENSP00000351141

    UniGene: Hs.446091